Characterisation of 16S rRNA Metagenomic Amplicon Data of Bacterial Communities Associated with IGR Treated Tropical Bed Bugs, Cimex hemipterus (F.) (Hemiptera)
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Abstract
Endosymbiont serves crucial physiological functions within their insect hosts, including nutrition provision, metabolic enhancement, and detoxification stimulation. The exploration of the microbiome population makeup corresponding to tropical bed bugs (Cimex hemipterus) became feasible with the advancement of novel metagenomic approaches. This study examined the microbial composition of tropical bed bugs treated with four different classes of insecticides: chlorfluazuron, tebufenozide, pyriproxyfen, β-cyfluthrin + imidacloprid, and control. Next, MiSeq Illumina was used to sequence the hypervariable section (v3-v4) of the 16S rRNA (ribosomal RNA) gene region. Proteobacteria accounted for over 99% of the overall microbial population in all samples. The two most common OTUs at the genus level were Wolbachia, an alpha-proteobacterium, and Pectobacterium, a gamma-proteobacterium. Despite some similarities in the major operational taxonomic units (OTUs) of the bacteria, the genera represented in the lower abundances were quite diverse. The bacterial abundance of bed bugs treated with pyriproxyfen exhibited a notable disparity from the control sample. This research lays the groundwork for a previously unknown symbiont-mediated mechanism influencing insecticide susceptibility in bed bugs.
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References
Akhoundi M, Cannet A, Loubatier C, Berenger J M, Izri A, Marty P and Delaunay P. (2016). Molecular characterization of Wolbachia infection in bed bugs (Cimex lectularius) collected from several localities in France. Parasite 23: 1–6. https://doi.org/10.1051/parasite/2016031
Amir R, Sani Q U A, Maqsood W, Munir F, Fatima N, Siddiqa A and Ahmad J. (2020). Pan-genomics of plant pathogens and its applications. In B Debmalya, S Siomar and A Vasco (eds.). Pan-genomics: Applications, challenges, and future prospects. London, United Kingdom: Academic Press, 121–145. https://doi.org/10.1016/B978-0-12-817076-2.00006-8
Bellinvia S, Johnston P R, Reinhardt K and Otti O. (2020). Bacterial communities of the reproductive organs of virgin and mated common bedbugs, Cimex lectularius. Ecological Entomology 45(1): 142–154. https://doi.org/10.1111/een.12784
Benoit J. B, Grosso, N A D, Yoder J. A and Denlinger D. L. (2007). Resistance to dehydration between bouts of blood feeding in the bed bug, Cimex lectularius, is enhanced by water conservation, aggregation, and quiescence. American Journal of Tropical Medicine and Hygiene 76(5): 987–993. https://doi.org/10.4269/ajtmh.2007.76.987
Boissière A, Tchioffo M T, Bachar D, Abate L, Marie A, Nsango S E and Morlais I. (2012). Midgut microbiota of the malaria mosquito vector Anopheles gambiae and interactions with Plasmodium falciparum infection. PLoS Pathogens 8(5): 1–12. https://doi.org/10.1371/journal.ppat.1002742
Bukin Y S, Galachyants Y P, Morozov I V, Bukin S V, Zakharenko A S and Zemskaya T I. (2019). The effect of 16S rRNA region choice on bacterial community metabarcoding results. Scientific Data 6(1): 1–14. https://doi.org/10.1038/sdata.2019.7
Doggett, S L, Dwyer D E, Penas P F and Russell R C. (2012). Bed bugs: Clinical relevance and control options. Clinical Microbiology Review 25: 164–192. https://doi.org/10.1128/CMR.05015-11
Doggett S L, Geary M J and Russell R C. (2004). The resurgence of bed bugs in Australia: With notes on their ecology and control. Environmental Health, 4(2): 30–38.
Dieng H, Satho T, Abang, F, Meli N K K B, Ghani I A, Nolasco-Hipolito C, Hakim, H, et al. (2017). Sweet waste extract uptake by a mosquito vector: Survival, biting, fecundity responses, and potential epidemiological significance. Acta Tropica 169: 84–92. https://doi.org/10.1016/j.actatropica.2017.0 1.022
Diouf M, Hervé V, Mora P, Robert A, Frechault S, Rouland-Lefèvre C and Miambi, E. (2018). Evidence from the gut microbiota of swarming alates of a vertical transmission of the bacterial symbionts in Nasutitermes arborum (Termitidae, Nasutitermitinae). Antonie van Leeuwenhoek 111(4): 573–587. https://doi.org/10.1007/s10482-017-0978-4
Dueholm M K D, Nierychlo M, Andersen K S, Rudkjøbing V, Knutsson S and Nielsen, P H. (2022). MiDAS 4: A global catalogue of full-length 16S rRNA gene sequences and taxonomy for studies of bacterial communities in wastewater treatment plants. Nature Communications 13(1): 1–15. https://doi.org/10.1038/s41467-022-29438-7
Evans N J, Brown J M, Murray R D, Getty B, Birtles R J, Hart C A and Carter S D. (2011). Characterization of novel bovine gastrointestinal tract treponema isolates and comparison with bovine digital dermatitis treponemes. Applied and Environmental Microbiology 77(1): 138–147. https://doi.org/1 0.1128/AEM.00993-10
Goddard J and DeShazo R. (2009). Bed bugs (Cimex lectularius) and clinical consequences of their bites. JAMA 301(13): 1358–1366. https://doi.org/10.1001/jama.2009.405
Goodman M H. (2016). Endosymbiotic bacteria in the bed bug, Cimex Lectularius L. (Hemiptera: Cimicidae). PhD diss., University of Kentucky.
Gupta S, Mortensen M S, Schjørring S, Trivedi U, Vestergaard G, Stokholm J, Bisgaard H, et al. (2019). Amplicon sequencing provides more accurate microbiome information in healthy children compared to culturing. Communications Biology 2(1): 1–7. https://doi.org/10.1038/s42003-019-0540-1
Harlan H J. (2006). Bed bugs 101: The basics of Cimex lectularius. American Entomologist 52(2): 99–101. https://doi.org/10.1093/ae/52.2.99
Heaton L L. (2013). Wolbachia in bedbugs Cimex lectularius. PhD diss., University of Sheffield.
Hickin M L, Kakumanu M L and Schal C. (2022). Effects of Wolbachia elimination and B-vitamin supplementation on bed bug development and reproduction. Scientific Reports 12(1): 1–14. https://doi.org/10.1038/s41598-022-14505-2
Hosokawa T, Koga R, Kikuchi Y, Meng X Y and Fukatsu T. (2010). Wolbachia as a bacteriocyte-associated nutritional mutualist. Proceedings of the National Academy of Sciences of the United States of America 107(2): 769–774. https://doi.org/10.1073/pnas.0911476107
Hugouvieux-Cotte-Pattat N. (2016). Metabolism and virulence strategies in dickeya–host interactions. In Progress in molecular biology and translational science. Netherlands: Academic Press, 93–129. https://doi.org/10.1016/bs.pmbts.2016.05.006
Hypša V and Aksoy S. (1997). Phylogenetic characterization of two transovarially transmitted endosymbionts of the bedbug Cimex lectularius (Heteroptera: Cimicidae). Insect Molecular Biology 6(3): 301–304. https://doi.org/10.1046/j.1365-2583.1997.00178.x
Kohl K D, Amaya J, Passement C A, Dearing M D and McCue M D. (2014). Unique and shared responses of the gut microbiota to prolonged fasting: A comparative study across five classes of vertebrate hosts. FEMS Microbiology Ecology 90(3): 883–894. https://doi.org/10.1111/1574-6941.12442
Li F, Li M, Zhu Q, Mao T, Dai M, Ye W, Bian D, et al. (2021). Imbalance of intestinal microbial homeostasis caused by acetamiprid is detrimental to resistance to pathogenic bacteria in Bombyx mori. Environmental Pollution 289: 1–11. https://doi.org/10.1016/j.envpol.2021.117866
Lim L and Majid A H A. (2021). Characterization of bacterial communities associated with blood-fed and starved tropical bed bugs, Cimex hemipterus (F.) (Hemiptera): A high throughput metabarcoding analysis. Scientific Reports 11(1): 1–12. https://doi.org/10.1038/s41598-021-87946-w
Lu Q, Li G, Lan H, Yu D, Yin X, Yang W, Yang Z, et al. (2022). Effects of exposure to trace pyriproxyfen on the intestinal bacterial diversity and immune signal pathways of silkworm (Bombyx mori) larvae. Journal of Asia-Pacific Entomology 25(2): 1–12. https://doi.org/10.1016/j.aspen.2022.101895
Mahenthiralingam E, Baldwin A and Dowson C G. (2008). Burkholderia cepacia complex bacteria: Opportunistic pathogens with important natural biology. Journal of Applied Microbiology 104(6): 1539–1551. https://doi.org/10.1111/j.1365-2672.2007.03706.x
Peta V and Pietri J E. (2021). Experimental infection of bed bugs (Cimex lectularius L.) with Burkholderia multivorans. Medical and Veterinary Entomology 35(3): 507–512. https://doi.org/10.1111/mve.12520
Pietri J E, Yax J A, Agany D D M, Gnimpieba E Z and Sheele J M. (2020). Body lice and bed bug co-infestation in an emergency department patient, Ohio, USA. IDCases 19: 1–4. https://doi.org/10.101 6/j.idcr.2020.e00696
Potter M F, Romero A, Haynes K F and Hardebeck E. (2007). Killing them softly: Battling bed bugs in sensitive places. Pest Control Technology 35: 24–32.
Rupawate P S, Roylawar P, Khandagale K, Gawande S, Ade A B, Jaiswal D K and Borgave S. (2023). Role of gut symbionts of insect pests: A novel target for insect-pest control. Frontiers in Microbiology 14: 1–14. https://doi.org/10.3389/fmicb.2023.1146390
Soh L S and Singham G V. (2022). Bacterial symbionts influence host susceptibility to fenitrothion and imidacloprid in the obligate hematophagous bed bug, Cimex hemipterus. Scientific Reports 12(1): 1–16. https://doi.org/10.1038/s41598-022-09015-0
Tye S R. (2023). Interplay between microbiome and temperature in Tenebrio molitor. Masters’ thesis, Texas State University.