Biological and Chemical Co-surfactant for Fabrication of Antibacterial Silver Nanoparticles and Potential Application in Agriculture

Main Article Content

Nguyen Nhat Nam

Abstract

Silver nanoparticles (AgNPs) have been widely applied as antimicrobial materials. In this work, a new fabrication method of AgNPs has been proposed through a combination of tea seed saponin extraction as a non-ionic biological surfactant and cetyltrimethylammonium chloride (CTAC) as a co-surfactant. The morphology and optical properties of as-prepared AgNPs were analysed by SEM and UV-vis absorbance measurement, respectively. The results indicate that AgNPs obtained high homogeneous particle sizes with a mean diameter of 44.5 ± 3.8 nm. The optical property of AgNPs was exhibited through a UV-vis absorbance spectrum of ~420 nm. In addition, the antibacterial behaviour of E. coli (ATCC 25922) was increased according to the AgNPs concentration. The diameter of inhibition zones was 12 mm, 14 mm and 16 mm under AgNPs concentrations of 0.8 ppm, 8 ppm and 80 ppm, respectively. Our initial trial treatment of AgNPs in young broccoli (Brassica oleracea) exhibited promising potential for plant protection in agricultural applications.

Article Details

How to Cite
Nguyen Nhat Nam. (2025). Biological and Chemical Co-surfactant for Fabrication of Antibacterial Silver Nanoparticles and Potential Application in Agriculture. Tropical Life Sciences Research, 36(3), 121-133. https://doi.org/10.21315/
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Original Article

References

Abdelgadir A, Adnan M, Patel M, Saxena J, Alam M J, Alshahrani M M, Singh R, Sachidanandan M, Badraoui R and Siddiqui A J. (2024). Probiotic Lactobacillus salivarius mediated synthesis of silver nanoparticles (AgNPs-LS): A sustainable approach and multifaceted biomedical application. Heliyon 10(18): e37987. https://doi.org/10.1016/J.HELIYON.2024.E37987

Ansari M, Ahmed S, Abbasi A, Hamad N A, Ali H M, Khan M T, Haq I U and Zaman Q u. (2023). Green synthesized silver nanoparticles: A novel approach for the enhanced growth and yield of tomato against early blight disease. Microorganisms 11(4): 886. https://doi.org/10.3390/MICROORGANISMS11040886

Bagheri A and Khalili P. (2017). Synergism between non-ionic and cationic surfactants in a concentration range of mixed monolayers at an air–water interface. RSC Advances 7(29), 18151–18161. https://doi.org/10.1039/C6RA27382C

Cunha F A, Maia K R, Mallman E J J, Cunha M D C D S O, Maciel A A M, De Souza I P, Menezes E A and Fechine P B A. (2016). Silver nanoparticles-disk diffusion test against Escherichia coli isolates. Revista Do Instituto de Medicina Tropical de São Paulo 58: 73. https://doi.org/10.1590/S1678-9946201658073

Dong S, Yang X, Zhao L, Zhang F, Hou Z and Xue P. (2020). Antibacterial activity and mechanism of action saponins from Chenopodium quinoa Willd. husks against foodborne pathogenic bacteria. Industrial Crops and Products 149: 112350. https://doi.org/10.1016/J.INDCROP.2020.112350

Ebrahiminezhad A, Taghizadeh S and Ghasemi Y. (2017). Green synthesis of silver nanoparticles using Mediterranean cypress (Cupressus sempervirens) leaf extract. American Journal of Biochemistry and Biotechnology 13(1): 1–6. https://doi.org/10.3844/AJBBSP.2017.1.6

Hemalatha M, Hilli J S, Chandrashekhar S S, Vijayakumar A G, Reddy U G and Tippannavar P S. (2024). Application of green synthesized Ag and Cu nanoparticles for the control of bruchids and their impact on seed quality and yield in greengram. Heliyon 10(11): e31551. https://doi.org/10.1016/j.heliyon.2024.e31551

Husain S, Nandi A, Simnani F Z, Saha U, Ghosh A, Sinha A, Sahay A, Samal S K, Panda P K and Verma S K. (2023). Emerging trends in advanced translational applications of silver nanoparticles: A progressing dawn of nanotechnology. Journal of Functional Biomaterials 14(1): 47. https://doi.org/10.3390/JFB14010047

Ivanov I, Manolov S, Phuong N, Nguyen U, Dang N T, Doan L, Thu T and Nguyen H. (2023). Synthesis of silver nanoparticles: From conventional to ‘modern’ methods: A review. Processes 11(9): 2617. https://doi.org/10.3390/PR11092617

Khan M I, Karima G, Khan M Z, Shin J H and Kim J D. (2022). Therapeutic effects of saponins for the prevention and treatment of cancer by ameliorating inflammation and angiogenesis and inducing antioxidant and apoptotic effects in human cells. International Journal of Molecular Sciences 23(18): 10665. https://doi.org/10.3390/IJMS231810665

Khan S, Zahoor M, Sher Khan R, Ikram M and Islam N U. (2023). The impact of silver nanoparticles on the growth of plants: The agriculture applications. Heliyon 9(6): e16928. https://doi.org/10.1016/J.HELIYON.2023.E16928

Khodeer D M, Nasr A M, Swidan S A, Shabayek S, Khinkar R M, Aldurdunji M M, Ramadan M A and Badr J M. (2023). Characterization, antibacterial, antioxidant, antidiabetic, and anti-inflammatory activities of green synthesized silver nanoparticles using Phragmanthera austroarabica A. G. Mill and J. A. Nyberg extract. Frontiers in Microbiology 13: 1078061. https://doi.org/10.3389/fmicb.2022.1078061

Krishnaraj C, Jagan E G, Rajasekar S, Selvakumar P, Kalaichelvan P T and Mohan N. (2010). Synthesis of silver nanoparticles using Acalypha indica leaf extracts and its antibacterial activity against water borne pathogens. Colloids and Surfaces B: Biointerfaces 76(1): 50–56. https://doi.org/10.1016/J.COLSURFB.2009.10.008

Liaqat N, Jahan N, Khalil-ur-Rahman, Anwar T and Qureshi H. (2022). Green synthesized silver nanoparticles: Optimization, characterization, antimicrobial activity, and cytotoxicity study by hemolysis assay. Frontiers in Chemistry 10: 952006. https://doi.org/10.3389/fchem.2022.952006

Menichetti A, Mavridi-Printezi A, Mordini D and Montalti M. (2023). Effect of size, shape and surface functionalization on the antibacterial activity of silver nanoparticles. Journal of Functional Biomaterials 14(5): 244. https://doi.org/10.3390/JFB14050244

Mikhailova E O. (2020). Silver nanoparticles: Mechanism of action and probable bio-application. Journal of Functional Biomaterials 11(4): 369. https://doi.org/10.3390/JFB11040084

More P R, Pandit S, Filippis A De, Franci G, Mijakovic I and Galdiero M. (2023). Silver nanoparticles: Bactericidal and mechanistic approach against drug resistant pathogens. Microorganisms 11(2): 369. https://doi.org/10.3390/MICROORGANISMS11020369

Rasheed A, Hussain S, Mushtaq W, Zubair M, Siddique K, Attia K, Khan N, Fiaz S, Azeem F and Chen Y. (2023). Application of silver nanoparticles synthesized through varying biogenic and chemical methods for wastewater treatment and health aspects. Environmental Science and Pollution Research 1: 1–18. https://doi.org/10.1007/s11356-022-24761-4

Salleh A, Naomi R, Utami N D, Mohammad A W, Mahmoudi E, Mustafa N and Fauzi M B. (2020). The potential of silver nanoparticles for antiviral and antibacterial applications: A mechanism of action. Nanomaterials 10(8): 1566. https://doi.org/10.3390/NANO10081566

Schreiner T B, Dias M M, Barreiro M F and Pinho S P. (2022). Saponins as natural emulsifiers for nanoemulsions. Journal of Agricultural and Food Chemistry 70(22): 6573–6590. https://doi.org/10.1021/acs.jafc.1c07893

Shoaib H, Mahesar S A, Sherazi S T H, Naz S, Ayyildiz H F, Sirajuddin, Memon H D and Sidhu A R. (2024). Evaluation of antioxidant, antibacterial, and anti-inflammatory activities of deodorizer distillate-derived silver nanoparticles. South African Journal of Chemical Engineering 50: 311–320. https://doi.org/10.1016/J.SAJCE.2024.09.002

Singh H, Kumar S and Arya A. (2024). Evaluation of antibacterial, antioxidant, and anti-inflammatory properties of GC/MS analysis of extracts of Ajuga. integrifolia Buch.-Ham. leaves. Scientific Reports 14(1): 1–13. https://doi.org/10.1038/s41598-024-67133-3

Yin I X, Zhang J, Zhao I S, Mei M L, Li Q and Chu C H. (2020). The antibacterial mechanism of silver nanoparticles and its application in dentistry. International Journal of Nanomedicine 15: 2555–2562. https://doi.org/10.2147/IJN.S246764

Yu X L and He Y. (2018). Optimization of tea‐leaf saponins water extraction and relationships between their contents and tea (Camellia sinensis) tree varieties. Food Science & Nutrition 6(6): 1734. https://doi.org/10.1002/FSN3.724

Yu X, Zhao Z, Yan X, Xie J, Yu Q and Chen Y. (2023). Extraction optimization of tea saponins from Camellia oleifera seed meal with deep eutectic solvents: Composition identification and properties evaluation. Food Chemistry 427: 136681. https://doi.org/10.1016/J.FOODCHEM.2023.136681

Zhang X, Li C, Hu W, Abdel-Samie M A, Cui H and Lin L. (2023). An overview of tea saponin as a surfactant in food applications. Critical Reviews in Food Science and Nutrition 64(33): 12922–12934. https://doi.org/10.1080/10408398.2023.2258392