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A wilt clove disease has been reported recently that triggered a widespread devastation among clove plantings in Central Java, Indonesia. In the early stage, the younger leaves showed wilt suddenly, falling from the twig tip of branches in the crown, leaving the twig tip bare. However, older leaves may also turned brown and stay attached to the branches of infected plants. Several causative pathogens have been previously reported; including oomycetes, fungi, and bacteria. The aim of this research was to confirm the pathogen and to characterize the disease from clove plantings in the field. The research involved on morphological characterization, histology of infected plants, and molecular characterization of pathogens based on the endoglucanase (egl) gene. Bacterial ooze was observed on the tip of infected stems, and bacterial colonisation of xylem tissue was observed in the infected plants by cross-sections histology comparison. The pathogenic strain, designated KD1, was isolated from diseased tissue by dilution method and confirmed by Koch’s postulates. It showed hypersensitive reaction positive on tobacco and pathogenic to host plants. Partial egl sequence analysis revealed that the pathogen was closely related to Ralstonia syzygii subsp. syzygii (causative agent of Sumatra disease of clove) with sequence similarity of > 99%.
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Ageichik A V, Evtushenkov A N and Nikolaichik Y A. (2002). The role of type III secretion system in Erwinia carotovora subsp. atroseptica virulence. Plant Protection Science 38(s2): 523–527.
Ausubel F M, Brent R, Kingston R E, Moore D D, Seidman J G, Smith J A and Struhl K. (2003). Current protocols in molecular biology. USA: John Wiley & Sons, Inc.,1600 pp.
Bennet C P A, Hunt P and Asman A. (1985). Association of a xylem-limited bacterium with Sumatra disease of cloves in Indonesia. Plant Pathology 34(4): 487–494. https://doi.org/10.1111/j.1365-3059.1985.tb01398.x
Bennet C P A, Jones P and Hunt P. (1987). Isolation, culture and ultrastructure of a xylemlimited bacterium associated with Sumatra disease of cloves. Plant Pathology 36(1): 45–52. https://doi.org/10.1111/j.1365-3059.1987.tb02176.x
Danaatmadja Y, Subandiyah S, Joko T and Sari C U. (2009). Isolation and characterization of Ralstonia syzygii. Jurnal Perlindungan Tanaman Indonesia 15: 7–12.
Dwimartina F, Arwiyanto T and Joko T. (2017). Potential of endophytic and rhizobacteria as an effective biocontrol for Ralstonia syzygii subsp. syzygii. Asian Journal of Plant Pathology 11: 191–198.
Eden-Green S J, Balfas R and Sutarjo T. (1992). Characteristics of the transmission of Sumatra disease of cloves by tube-building cercopoids, Hindola spp. Plant Pathology 41(6): 702–712. https://doi.org/10.1111/j.1365-3059.1992.tb02553.x
Fegan M and Prior P. (2005). How complex is the Ralstonia solanacearum species complex?. In: C Allen, P Prior and A C Hayward (eds.), Bacterial wilt disease and the Ralstonia solanacearum species complex. St. Paul, MN: APS Press, 449–461.
Gitaitis, R D. (1990). Induction of hypersensitive like reaction in four o’clock by Clavibacter michiganensis ssp. michiganensis. Plant Disease 74: 58–60. https://doi.org/10.1094/PD-74-0058
Hidayah N, Abadi A L and Aini L Q. (2014). Morphological and physiological characterization of Ralstonia syzygii from clove. Journal of Agriculture and Veterinary Science 8(7): 87–91.
Ismiyatuningsih, Joko T and Hartono S. (2016). Survey and detection of Pectobacterium atrosepticum in major potato-growing areas in Central Java Province, Indonesia. Agricultural Science 1: 1–6.
Joko T, Subandiyah S and Somowiyarjo S. (2000). The role of extracellular protein on the pathogenicity of Xanthomonas campestris pv. citri. Jurnal Perlindungan Tanaman Indonesia 6: 32–38.
Joko T, Hirata H and Tsuyumu S. (2007a). Sugar transporter (MfsX) of major facilitator superfamily is required for flagella-mediated pathogenesis in Dickeya dadantii 3937. Journal of General Plant Pathology 73(4): 266–273. https://doi.org/10.1007/s10327-007-0018-8
___. (2007b). A sugar transporter (MfsX) is also required by Dickeya dadantii 3937 for in planta fitness. Journal of General Plant Pathology 73(4): 274–280.
Joko T, Koentjoro M P, Somowiyarjo S, Rohman M S, Liana A and Ogawa N. (2012). Response of rhizobacterial communities in watermelon to infection with cucumber green mottle mosaic virus as revealed by cultivation-dependent RISA. Archives of Phytopathology and Plant Protection 45(15): 1810–1818. https://doi.org/10.1080/03235408.2012.707526
Joko T, Subandi A, Kusumandari N, Wibowo A and Priyatmojo A. (2014). Activities of plant cell wall-degrading enzymes by bacterial soft rot of orchid. Archives of Phytopathology and Plant Protection 47(10): 1239–1250. https://doi.org/10.1080/03235408.2013.838374
Joko T, Umehara M, Murata T, Etoh H, Izumori K and Tsuyumu S. (2018). Hyperinduction of pectate lyase in Dickeya chrysanthemi EC16 by plant-derived sugars. Journal of Plant Interactions 13(1): 141–150. https://doi.org/10.1080/17429145.2018.1444206
Klement Z and Goodman R N. (1967). The hypersensitive reaction to infection by bacterial plant pathogens. Annual Review of Phytopathology 5: 17–44. https://doi.org/10.1146/annurev.py.05.090167.000313
Lindgren P B. (1997). The role of hrp genes during plant bacterial interactions. Annual Review of Phytopathology 35: 129–152. https://doi.org/10.1146/annurev.phyto.35.1.129
Mahfut, Joko T and Daryono B S. (2016). Molecular characterization of odontoglossum ring spot virus (ORSV) in Java and Bali, Indonesia. Asian Journal of Plant Pathology 10(1–2): 9–14.
Nurjanah N, Joko T and Subandiyah S. (2017). Characterization of Pantoea ananatis isolated from garlic and shallot. Jurnal Perlindungan Tanaman Indonesia 21: 120–126.
Roberts S J, Eden-Green S J, Jones P and Ambler D J. (1990). Pseudomonas syzygii, sp. nov., the cause of Sumatra disease of cloves. Systematic and Applied Microbiology 13(1): 34–43. https://doi.org/10.1016/S0723-2020(11)80178-5
Safni I, Subandiyah S and Fegan M. (2018). Ecology, epidemiology and disease management of Ralstonia syzygii in Indonesia. Frontiers in Microbiology 9: 1–11. https://doi.org/10.3389/fmicb.2018.00419
Suharti T, Joko T and Arwiyanto T. (2017). Detection of seed-borne pathogenic bacteria of northern black wattle (Acacia auriculiformis A. Cunn. ex Benth.). Jurnal Hama dan Penyakit Tumbuhan Tropika 17: 19–36.
Sutikno. (2016). Plant microtechnique. Yogyakarta: Universitas Gajah Mada Press. pp. 51. Sutrisno, Susanto F A, Wijayanti P, Retnoningrum M D, Nuringtyas T R, Joko T and Purwestri Y A. (2018). Screening of resistant Indonesian black rice cultivars against bacterial leaf blight. Euphytica 214: 199. https://doi.org/10.1007/s10681-018-2279-z
Tortora G J, Funke B R and Case C L. (2010). Microbiology: An introduction (10thEd.). San Francisco: Benjamin Cummings, pp. 812.
Trianom B, Arwiyanto T and Joko T. (2018). Development of novel subspecies-specific primers based on the endoglucanase gene for detection of Ralstonia syzygii subsp. syzygii. Jurnal Perlindungan Tanaman Indonesia 22: 124–131.
Vaneechoutte M, Kampfer P, Baere T D, Falsen E and Verschraegen G. (2004). Wautersia gen. nov., a novel genus accommodating the phylogenetic lineage including Ralstonia eutropha and related species, and proposal of Ralstonia [Pseudomonas] syzygii. International Journal of Systematic and Evolutionary Microbiology 54(2): 317–327.
Waller J M and Sitepu D. (1975). Sumatra disease of cloves in Indonesia. Pest Articles and News Summaries 21(2): 141–147.
Widyaningsih S, Utami S N H, Joko T and Subandiyah S. (2017). Development of disease and growth on six scion/root stock combinations of citrus seedlings under huanglongbing pressure. Journal of Agricultural Science 9(6): 229–238.
Windari U, Joko T and Subandiyah S. (2015). Detection of bacterial fruit blotch of melon using ELISA. Jurnal Perlindungan Tanaman Indonesia 19(1): 1–5.