The Optimal Dosage of Fermented Herbal Extract on Growth and Feed Efficiency of Nile Tilapia (Oreochromis niloticus)

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Yushinta Fujaya
Andi Aliah Hidayani
Dwi Kesuma Sari
Siti Aslamyah
Nita Rukminasari
Abdul Muthalib
Stevie Cristianto
Emilia Defista
Hanafiah Fazhan
Khor Waiho


Owing to their availability, cost effectiveness and environmental-friendly nature, plant extracts are promising additives for fish farming. This study aims to determine the optimal dosage of fermented herbal extract (FHE)—composed of Morus alba (33.3%), Curcuma xanthorrhiza (33.3%), and Boesenbergia rotunda (33.3%)—for growth enhancement and feed utilisation efficiency of Oreochromis niloticus fingerlings. Fermentation was conducted using probiotics Lactobacillus casei (Yakult®, Tokyo, Japan) and Saccharomyces cereviceae (commercial baker’s yeast). The FHE was high in flavonoid and alkaloid, vitamin C, potassium, natrium, lipase and protease. Four doses of FHE treatments, namely treatment A (0 mg/kg of feed); treatment B (100 mg/kg of feed); treatment C (300 mg/kg of feed); treatment D (500 mg/kg of feed) were compared. After subjected to 35 days of culture, tilapias subjected to FHE-coated feed exhibited better weight gain (WG), specific growth rate (SGR), and feed efficiency (FE) compared to control. The best dosage that gave the highest growth and feed efficiency was treatment C (300 mg/kg of feed). Furthermore, the feed efficiencies of FHE-incorporated treatments were positively influenced by the increased in length and density of intestinal villi, number of goblet cells, lymphocytes, as well as nutrient retention to support growth. The results of this study indicate that FHE is a promising functional feed additive to stimulate growth and improve feed efficiency in tilapia farming.

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The Optimal Dosage of Fermented Herbal Extract on Growth and Feed Efficiency of Nile Tilapia (Oreochromis niloticus) . (2023). Tropical Life Sciences Research, 34(2), 39–56.
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Abdel-Tawwab M, Ahmad M, Seden M E A and Sakr S F M. (2010). Use of green tea, Camellia sinensis L., in practical diet for growth and protection Nile tilapia, Oreochromis niloticus (L.)., against Aeromonas hydrophila infection. Journal of the World Aquaculture Society 41(2): 203–2013. 7345.2010.00360.x

Anders N, Roth B, Grimsbø E and Breen M. (2019). Assessing the effectiveness of an electrical stunning and chilling protocol for the slaughter of Atlantic mackerel (Scomber scombrus). PLoS ONE 14(9): e0222122. pone.0222122

Ashry A M, Hassan A M, Habiba M M, El-zayat A, El-sharnouby M E, Sewilam H and Dawood M A O. (2021). The impact of dietary curcumin on the growth performance, intestinal antibacterial capacity, and haemato-biochemical parameters of gilthead seabream (Sparus aurata). Animals 11(6): 1779.

Bertucci J I, Blanco A M, Sundarranjan L, Rajeswari J J, Velasco C and Unniappan S. (2019). Nutrient regulation of endocrine factors influencing feeding and growth in fish. Frontiers in Endocrinology 10: 83.

Birchenough G M H, Johansson M E V, Gustafsson J K, Bergström J H and Hansson G C. (2015). New developments in goblet cell mucus secretion and function. Mucosal Immunology 8(4): 712–719.

Booman M, Foster I, Vederas J C, Groman D B and Jones S R M. (2018). Soybean meal-induced enteritis in Atlantic salmon (Salmo salar) and Chinook salmon (Oncorhynchus tshawytscha) but not in pink salmon (O. gorbuscha). Aquaculture 483: 238–243.

Bosi G, Giari L, DePasquale J A, Carosi A, Lorenzoni M and Sayyaf Dezfuli B. (2017). Protective responses of intestinal mucous cells in a range of fish-helminth systems. Journal of Fish Diseases 40(8): 1001–1014.

Boyd C E and McNevin A A. (2014). Chemicals in aquaculture. In: Boyd C E and McNevin A A (eds.), Aquaculture, resource use, and the environment. New Jersey: John Wiley and Sons, 173–210.

Chen C, Razali U H M, Saikim F H, Mahyudin A and Noor N Q I M. (2021). Morus alba L. plant: Bioactive compounds and potential as a functional food ingredient. Foods 10(3): 689.

Chitmanat C, Lebel P, Whangchai N, Promya J and Lebel L. (2016). Tilapia diseases and management in river-based cage aquaculture in northern Thailand. Journal of Applied Aquaculture 28(1): 9–16.

Cho C Y and Bureau D P. (1995). Determination of the energy requirements of fish with particular reference to salmonids. Journal of Applied Ichthyology 11(3–4): 141– 163.

Das N, Mishra S K, Bishayee A, Ali E S and Bishayee A. (2021). The phytochemical, biological, and medicinal attributes of phytoecdysteroids: An updated review. Acta Pharmaceutica Sinica B 11(7): 1740–1766. apsb.2020.10.012

Elfahmi, Woerdenbag H J and Kayser O. (2014). Jamu: Indonesian traditional herbal medicine towards rational phytopharmacological use. Journal of Herbal Medicine 4(2): 51–73.

Eng-Chong T, Yean-Kee L, Chin-Fei C, Choon-Han H, Sher-Ming W, Li-Ping C T, Gen- Teck F, Khalid N, Rahman N A, Karsani, S A, Othman S, Othman R, et al. (2012). Boesenbergia rotunda: From ethnomedicine to drug discovery. Evidence- Based Complementary and Alternative Medicine 2012: 473637. https://doi. org/10.1155/2012/473637

FAO. (2021). GLOBEFISH-Information and analysis on world fish trade. Major tilapia markets weaken as supply increases. market-reports/resource-detail/en/c/1268646/

_______. (2020a). The state of world fisheries and aquaculture 2020. Sustainability in action. Rome: FAO.

_______. (2020b). Understanding antimicrobial resistance in aquaculture. Rome: FAO.

Fracalossi D M, Allen M E, Yuyama L K and Oftedal O T. (2001). Ascorbic acid biosynthesis in Amazonian fishes. Aquaculture 192: 321–332. 8486(00)00455-5

Francis G, Makkar H P S and Becker K. (2001). Antinutritional factors present in plant-derived alternate fish feed ingredients and their effects in fish. Aquaculture 199 (3–4): 197–227.

Fujaya Y, Trijuno D D, Rusdi M and Usman Z. (2018). Effectivity of mulberry leaf extract on stimulating ekdisteroid hemolimph content and molting of mud crab (Scylla olivacea). Torani 2(1): 32–43.

Gabriel N N. (2019). Review on the progress in the role of herbal extracts in tilapia culture. Cogent Food and Agriculture 5(1): 1619651. 19.1619651

Gabriel N N, Qiang J, Ma X Y, He J, Xu P and Liu K. (2015). Dietary Aloe vera improves plasma lipid profile, antioxidant, and hepatoprotective enzyme activities in GIFT-tilapia (Oreochromis niloticus) after Streptococcus iniae challenge. Fish Physiology and Biochemistry 41(5): 1321–1332.

Goda A M A S. (2008). Effect of dietary ginseng herb (Ginsana ® G115) supplementation on growth, feed utilization, and hematological indices of Nile tilapia, Oreochromis niloticus (L.), fingerlings. Journal of the World Aquaculture Society 39(2): 205–214.

Gorelick-Feldman H, Cohick W and Raskin I. (2010). Ecdysteroids elicit a rapid Ca2+ flux leading to Akt activation and increased protein synthesis in skeletal muscle cells. Steroids 75(10): 632–637.

Guo H and Dixon B. (2021). Understanding acute stress-mediated immunity in teleost fish. Fish and Shellfish Immunology Reports 2: 100010. fsirep.2021.100010

Hardi E H, Nugroho R A, Rostika R, Mardliyaha C M, Sukarti K, Rahayu W, Supriansyah A and Saptiani G. (2022). Synbiotic application to enhance growth, immune system, and disease resistance toward bacterial infection in catfish (Clarias gariepinus). Aquaculture 549: 737794.

Hardi S H, Kusuma I W, Suwinarti W, Saptiani G, Sumoharjo and Lusiastuti A M. (2017). Utilization of several herbal plant extracts on Nile tilapia in preventing Aeromonas hydrophila and Pseudomonas sp. bacterial infection. Nusantara Bioscience 9(2): 220–228. Hvas M, Nilsen T O and Oppedal F. (2018). Oxygen uptake and osmotic balance of Atlantic salmon in relation to exercise and salinity acclimation. Frontiers in Marine Science 5: 368. Ibrahim R E, Ahmed S A A, Amer S A, Al-Gabri N A, Ahmed A I, Abdel-Warith A A, Younis E M I and Metwally A E. (2020). Influence of vitamin C feed supplementation on the growth, antioxidant activity, immune status, tissue histomorphology, and disease resistance in Nile tilapia, Oreochromis niloticus. Aquaculture 192: 321–332. https://

Kareem Z H, Abdelhadi Y M, Christianus A, Karim M and Romano N. (2016). Effects of some dietary crude plant extracts on the growth and gonadal maturity of Nile tilapia (Oreochromis niloticus) and their resistance to Streptococcus agalactiae infection. Fish Physiology and Biochemistry 42(2): 757–769. 015-0173-3

Kaur J, Kaur S and Mahajan A. (2013). Herbal medicines: Possible risks and benefits. American Journal of Phytomedicine and Clinical Therapeutics 1(2): 226–239.

Kumar V, Sinha A K, Makkar H P S, De Boeck G and Becker K. (2012). Phytate and phytase in fish nutrition. Journal of Animal Physiology and Animal Nutrition 96(3): 335–364.

Latif M, Faheem M, Asmatullah, Hoseinifar S H and Doan H V. (2021). Dietary black seed effects on growth performance, proximate composition, antioxidant and histo-biochemical parameters of a culturable fish, rohu (Labeo rohita). Animals 11(1): 48.

Lynch A J, Elliott V, Phang S C, Claussen J E, Harrison I, Murchie K J, Steel E A and Stokes G L. (2020). Inland fish and fisheries integral to achieving the Sustainable Development Goals. Nature Sustainability 3: 579–587. s41893-020-0517-6

Ma H, Tao W and Zhu S. (2019). T lymphocytes in the intestinal mucosa: Defense and tolerance. Cellular and Molecular Immunology 16(3): 216–224. https://doi. org/10.1038/s41423-019-0208-2

Moh J H Z, Waiho K, Fazhan H, Shaibani N, Manan H, Sung Y Y, Ma H and Ikhwanuddin M. (2021). Effect of noni, Morinda citrifolia fruit extract supplementation on the growth performances and physiological responses of the hepatopancreas of Whiteleg shrimp, Penaeus vannamei post larvae. Aquaculture Reports 21: 100798. https://

Obirikorang K A, Gyamfi S, Goode M E, Amisah S, Edziyie R E, Quagrainie K, Egna H and Frimpong E. (2020). Effect of soybean meal diets on the growth performance, ammonia excretion rates, gut histology and feed cost of Nile tilapia (Oreochromis niloticus) fry. Aquaculture Research 51(9): 3520–3532. are.14689

Oon S F, Nallappan M, Tee T T, Shohaimi S, Kassim N K, Sa’ariwijaya M S F and Cheah Y H. (2015). Xanthorrhizol: A review of its pharmacological activities and anticancer properties. Cancer Cell International 15: 100. 0255-4

Palipoch S, Jiraungkoorskul W, Tansatit T, Preyavichyapugdee N, Jaikua W and Kosai, P. (2011). Protective efficiency of Thunbergia laurifolia leaf extract against lead (II) nitrate-induced toxicity in Oreochromis niloticus. Journal of Medicinal Plants Research 5(5): 719–728.

Poolsawat L, Yu Y, Li X, Zhen X, Yao W, Wang P, Luo C and Leng X. (2020). Efficacy of phytogenic extracts on growth performance and health of tilapia (Oreochromis niloticus × O. aureus). Aquaculture and Fisheries 7: 411–419. https://doi. org/10.1016/j.aaf.2020.08.009

Samtiya M, Aluko R E, Puniya A K and Dhewa T. (2021). Enhancing micronutrients bioavailability through fermentation of plant-based foods: A concise review. Fermentation 7: 63.

Siddik MAB, Foysal MJ, Fotedar R, Francis DS, Gupta SK (2022). Probiotic yeast Saccharomyces cerevisiae coupled with Lactobacillus casei modulates physiological performance and promotes gut microbiota in juvenile barramundi, Lates calcarifer. Aquaculture 546: 737346. aquaculture.2021.737346

Sklan D, Prag T and Lupatsch I. (2004). Structure and function of the small intestine of the tilapia Oreochromis niloticus x Oreochromis aureus (Teleostei, Cichlidae). Aquaculture Research 35(4): 350–357. 2109.2004.01020.x

Sohrabnezhad M, Sudagar M and Mazandarani M. (2017). Effect of dietary soybean meal and multienzyme on intestine histology of beluga sturgeon (Huso huso). International Aquatic Research 9: 271–280. 0172-3

Sokrab A M, Mohamed Ahmed I A and Babiker E E. (2014). Effect of fermentation on antinutrients, and total and extractable minerals of high and low phytate corn genotypes. Journal of Food Science and Technology 51(10): 2608–2615. https://

Suphoronski S A, Chideroli R T, Facimoto C T, Mainardi R M, Souza F P, Lopera-Barrero N M, Jesus G F A, Martins M L, Di Santis G W, de Oliveira A, et al. (2019). Effects of a phytogenic, alone and associated with potassium diformate, on tilapia growth, immunity, gut microbiome and resistance against francisellosis. Scientific Reports 9(1): 6045.

Tran N, Shikuku K M, Rossignoli C M, Barman B K, Cheong K C, Ali M S and Benzie J A H. (2021). Growth, yield and profitability of genetically improved farmed tilapia (GIFT) and non-GIFT strains in Bangladesh. Aquaculture 536: 736486. https://doi. org/10.1016/j.aquaculture.2021.736486

Waiho K, Afiqah-Aleng N, Iryani M T M and Fazhan H. (2021a). Protein-protein interaction network: an emerging tool for understanding fish disease in aquaculture. Reviews in Aquaculture 13(1): 156–177.

Waiho K, Ikhwanuddin M, Baylon J C, Jalilah M, Rukminasari N, Fujaya Y and Fazhan H. (2021b). Moult induction methods in soft-shell crab production. Aquaculture Research 52(9): 4026–4042.

Wang Y, Wang L, Zhang C and Song K. (2017). Effects of substituting fishmeal with soybean meal on growth performance and intestinal morphology in orange-spotted grouper (Epinephelus coioides). Aquaculture Reports 5: 52–57. aqrep.2016.12.005

Wu Q, Jiang Y, Chen E, Mu C and Waiho K. (2021). Chinese gallnut (Galla chinensis) against Vibrio parahaemolyticus: In vitro activity and the use of medicated bath method to treat infected mud crab Scylla paramamosain. Aquaculture 539: 736632. https://

Zhang J, Onakpoya I J, Posadzki P and Eddouks M. (2015). The safety of herbal medicine: From prejudice to evidence. Evidence-Based Complementary and Alternative Medicine 2015: 316706.