Silver Nanoparticles for Enhancing the Efficiency of Micropropagation of Banana (Musa acuminata L.)

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Samih M. Tamimi
Halima Othman


Silver nanoparticles (AgNPs) have numerous applications in plant biotechnology. The unique biological activities of AgNPs in reducing microbial contamination and promoting in vitro plant growth have encouraged their use in the development of novel culture systems for the in vitro cultivation of several plant species. In this study, the influence of (80 nm–100 nm) AgNPs on the micropropagation of banana was examined by incorporating AgNPs into shoot multiplication and rooting media at concentrations of 3 mg/L–15 mg/L. Biometric parameters for shoot multiplication (number of shoots/explant, shoot length and leaf surface area) and root development (number of roots/explant and root length) were analysed. In addition, shoot chlorophyll content, proline content and the possible impact of lipid peroxidation on membrane stability of plantlets were estimated. The results showed that all concentrations of AgNPs stimulated shoot growth and enhanced root development. The highest response was observed in media supplemented with 12 mg/L AgNPs. This optimal level of AgNPs caused a threefold increase in shoot growth parameter and a similar increase in root numbers/shoot and root length. Treatment with AgNPs at 12 mg/L also increased chlorophyll and proline content of shoots by 25% and 120% over control, respectively. Although the application of AgNPs increased the level of lipid peroxidation in shoots, it however, had a limited influence on membrane stability index. These results suggested that the administration of AgNPs to culture media can be effectively utilised for the enhancement of banana micropropagation with minimal toxic effects.

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How to Cite
Samih M. Tamimi, & Halima Othman. (2023). Silver Nanoparticles for Enhancing the Efficiency of Micropropagation of Banana (Musa acuminata L.). Tropical Life Sciences Research, 34(2), 161–175.
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Aghdaei M, Salehi, H and Sarmast M K. (2012). Effects of silver nanoparticles on Tecomella undulata (Roxb.) seem micropropagation. Advances in Horticulture Science 26(1): 21–24.

Barbasz A, Kreczmer B and O?wieja M. (2016). Effects of exposure of callus cells of two wheat varieties to silver nanoparticles and silver salt (AgNO3). Acta Physiologiae Plantarium 38: 76.

Bates L S, Walden R P and Teare I D. (1973). Rapid determination of proline for water stress studies. Plant Soil 39: 205–207.

Bello-Bello J J, Chavez-Santoscoy R A, Lecona-Guzmán C A, Bogdanchikova N, Salinas- Ruíz J, Gómez-Merino F C and Pestryakov A. (2017). Hormetic response by silver nanoparticles on in vitro multiplication of sugarcane (Saccharum spp. Cv. Mex 69–290) using a temporary immersion system. Dose Response 15(4): 1–9. https://

Castro-González C G, Sánchez-Segura L, Gómez-Merino F C and Bello-Bello J. (2019). Exposure of stevia (Stevia rebaudiana B.) to silver nanoparticles in vitro: Transport and accumulation. Scientific Reports 9: 10372. 019-46828-y

Duncan D B. (1955). Multiple range and multiple f-test. Biometrics 11(1): 1–5. https://doi. org/10.2307/3001478

El-Mahdy M T K, Radi A A and Shaaban M M. (2019). Impacts of exposure of banana to silver nanoparticles and sliver ions in vitro. Middle East Journal of Applied Science 9(3): 727–740.

El-Kosary S, Allatif A A, Stino R, Hassan M and Kinawy A A. (2020). Effect of silver nanoparticles on micropropagation of date palm (Phoenix dactylifera L., Cv. Sewi and Medjool). Plant Archives 20(2): 9701–9706.

Eustache T A E, Agbidinoukoun A A, Zandjanakou-Tachin M, Cacai G T H and Ahanhanzo C. (2021). Mass production of bananas and plantains (Musa spp.) plantlets through in vitro tissue culture partway: A review. European Journal of Biology and Biotechnology 2(4): 1–8.

Geisler-Lee J, Qiang W, Ying Y, Wen Z, Matt G, Kungang L, Ying H, Yongsheng C, Andrei K and Xingmao M. (2012). Phytotoxicity, accumulation and transport of silver nanoparticles by Arabidopsis thaliana. Nanotoxicology 7(3): 323–337. https://doi. org/10.3109/17435390.2012.658094

Gorka D E , Osterberg J S , Gwin C A , Colman B P, Meyer J N , Bernhardt E S, Gunsch C K, DiGulio R T and Liu J. (2015). Reducing environmental toxicity of silver nanoparticles through shape control. Environmental Science & Technology 49(16): 10093–10098.

Gupta S D, Agarwal A. and Pradhan S. (2018). Phytostimulatory effect of silver nanoparticles (AgNPs) on rice seedling growth: An insight from antioxidative enzyme activities and gene expression patterns. Ecotoxicology and Environmental Safety 161: 624– 633.

Ha N T M, Manh Do C, Hoang T T , Nghiep D, LeVan B. and Duong T. (2020).The effect of cobalt and silver nanoparticles on overcoming leaf abscission and enhanced growth of rose (Rosa hybrida L. ‘Baby Love’) plantlets cultured in vitro. Plant Cell, Tissue and Organ Culture 141: 393–405. 01796-4

Homaee B M and Ehsanpour A A.(2015). Physiological and biochemical responses of potato (Solanum tuberosum) to silver nanoparticles and silver nitrate treatments under in vitro conditions. Indian Journal of Plant Physiology 20: 353–359. https://

Huong B T T, Xuan T D, Trung K H, Ha T T, Duong V X, Khanh T D. and Gioi D H. (2021). Influences of silver nanoparticles in vitro morphogenesis of specialty King Banana (Musa spp.). Vietnam. Plant Cell Biotechnology and Molecular Biology 22(33–34): 163–175.

Kong I C, Ko K S and Koh D C. (2020). Evaluation of the effects of particle sizes of silver nanoparticles on various biological systems. International Journal of Molecular Sciences 21(22): 8465.

Kumari M, Pandey S, Bhattacharya A, Mishra A and Nautiyal C. (2017). Protective role of biosynthesized silver nanoparticles against early blight disease in Solanum lycopersicum. Plant Physiology and Biochemistry 121: 216–225 https://doi. org/10.1016/j.plaphy.2017.11.004

Lichtenthaler H K. (1987). Chlorophylls and carotenoids: Pigments of photosynthetic biomembranes. Methods in Enzymology 148: 350–382. https://doi. org/10.1016/0076-6879(87)48036-1

Lutts S, Kinet J M and Bouharmont J. (1996). NaCl-induced senescence in leaves of rice (Oryza sativa L.) cultivars differing in salinity resistance. Annals of Botany 78(3): 389–398.

McShan D, Ray P C and Yu H. (2014). Molecular toxicity mechanism of nanosilver. Journal of Food and Drug Analysis 22(1): 116–127. jfda.2014.01.010

Mirzajani F, Askari H, Hamzelou S, Farzaneh M, and Ghassempour A. (2013). Effect of silver nanoparticles on Oryza sativa L. and its rhizosphere bacteria. Ecotoxicology and Environmental Safety 88: 48–54.

Murashige T and Skoog F A. (1962). A revised medium for rapid growth and bioassays with tobacco tissue cultures Physiologia Plantarum 15(3): 473–479. https://doi. org/10.1111/j.1399-3054.1962.tb08052.x

Qian H, Peng X, Han X, Ren J, Sun L and Zhengwei F. (2013). Comparison of the toxicity of silver nanoparticles and silver ions on the growth of terrestrial plant model Arabidopsis thaliana. Journal of Environmental Sciences 25(9): 1947–1956.

Rahmawati, M, Choirul, M, Gianfranco R and Jadid N. (2022). Nanotechnology in plant metabolite improvement and in animal welfare. Applied Sciences 12(2): 838.

Ruffini Castiglione M and Cremonini R. (2009). Nanoparticles and higher plants. Caryologia 62(2): 161–165

Sairam R K, Deshmukh P S and Sukla D S. (1997). Tolerence to drought and temperature stress in relation to increased antioxidant enzyme activity in wheat. Journal of Agronomy and Crop Science 178(3): 171–177. 037X.1997.tb00486.x

Shaikhaldein H O, Al-Qurainy F, Nadeem M, Khan S, Tarroum M and Salih A. (2020). Biosynthesis and characterization of silver nanoparticles using Ochradenus arabicus and their physiological effect on Maerua oblongifolia raised in vitro. Scientific Reports 10: 17569.

Spinoso-Castillo J L., Chavez-Santoscoy R A, Bogdanchikova N, Pérez-Sato J A, Morales- Ramos V and Bello-Bello J J. (2017). Antimicrobial and hermetic effects of silver nanoparticles on in vitro regeneration of vanilla (Vanilla planifolia Jacks. ex Andrews) using a temporary immersion system. Plant Cell, Tissue and Organ Culture 129: 195–207.

Stewart R C and Bewley J D. (1980). Lipid peroxidation associated with accelerated aging of soybean axes. Plant Physiology 65(2): 245–248. pp.65.2.245

Su L J, Zhang J H, Gomez H, Murugan R, Hong X, Xu D, Jiang F and Peng Z Y. (2019). Reactive oxygen species-induced lipid peroxidation in apoptosis, autophagy, and ferroptosis. Oxidative Medicine and Cellular Longevity 2019 (Special Issue): 5080843.

Tabaei-Aghdaei S, Harrison P and Pearee R S. (2000). Expression of dehydration-stress related genes in crown of wheat grass species having contrasting acclimation to salt, cold and drought. Plant, Cell and Environment 23: 561–571. https://doi. org/10.1046/j.1365-3040.2000.00572.x

Tung H T, Bao H G, Cuong D M, Ngan H T M, Hien V T, Luan V Q, Vinh B V T, Phuong H T N, Nam N B, Trieu L N, Truong N K, Hoang P N D and Nhut D T. (2021). Silver nanoparticles as the sterilant in largescale micropropagation of chrysanthemum. In Vitro Cellular & Developmental Biology – Plant 57: 897–906. https://doi. org/10.1007/s11627-021-10163-7

Vinkovi? T, Novák O, Strnad M, Goessler W, Jurašin D D, Para?ikovi? N and Vr?ek I V. (2017). Cytokinin response in pepper plants (Capsicum annuum L.) exposed to silver nanoparticles. Environmental Research 156: 10–18. envres.2017.03.015

Zuverza-Mena N, Armendariz R, Peralta-Videa J R and Gardea-Torresdey J L. (2016). Effects of silver nanoparticles on radish sprouts: Root growth reduction and modifications in the nutritional value. Frontiers in Plant Science 7: 90. https://doi. org/.3389/fpls.2016.00090