Seminal Plasma Proteins Associated with The Fertility of Brahman Bulls in The Colombian Low Tropics
Main Article Content
Abstract
The sperm interacts with seminal plasma proteins during its transport through the female reproductive tract to reach the oocyte. Seminal plasma proteins have been associated as biomarkers of fertility in bovine males, while two-dimensional electrophoresis in polyacrylamide gels under denaturing conditions (2D-PAGE) is a useful technique for their separation, allowing their subsequent analysis with the aid of specialized software. Brahman bulls are known for their tolerance to tropical conditions such as low-quality pastures, high temperatures, and relative humidity as well as moderate resistance to infestations by parasites and insects. The present study describes the two-dimensional electrophoretic profiles of the seminal plasma proteins in the rainy and dry seasons, associating them with the fertility of Brahman bulls in the Colombian Orinoquía in a 90-days breeding season and a single-sire mating system (1 bull per 50 Brahman cows) with 60 consecutive days of rest. The fertility-related seminal plasma protein spots increased in the dry season. Likewise, a meaningful relationship was found between the protein spots that possibly coincide with the Binder of Sperm Proteins. It was also found that bulls with the highest percentages of pregnancy also had similarities in their 2D seminal plasma maps. We conclude that the seminal plasma protein profile of Brahman bulls raised in the Colombian low tropic changes between rainy and dry seasons, and such changes may influence the reproductive performance of those animals.
Article Details
This work is licensed under a Creative Commons Attribution 4.0 International License.
References
Amann R P. (1989). Can the fertility potential of a seminal sample be predicted accurately? Journal of Andrology 10(2): 89–98. https://doi.org/10.1002/j.1939-4640.1989.tb00066.x
Arora P S, Yamagiwa H, Srivastava A, Bolander M E and Sarkar G. (2005). Comparative evaluation of two two-dimensional gel electrophoresis image analysis software applications using synovial fluids from patients with joint disease. Journal of Orthopaedic Science 10(2): 160–166. https://doi.org/10.1007/s00776-004-0878-0
Azevedo M, M F A, Saturnino H M, Lana A M Q, Sampaio I B M, Monteiro J B N and Morato L E. (2005). Estimativa de níveis críticos superiores do índice de temperatura e umidade para vacas leiteiras 1/2, 3/4 e 7/8 holandês-zebu em lactação. Revista Brasileira de Zootecnia 34: 2000–2008. https://doi.org/10.1590/S1516-35982005000600025
Bohmanova J, Misztal I and Cole J B. (2007). Temperature-humidity indices as indicators of milk production losses due to heat stress. Journal of Dairy Science 90(4): 1947– 1956. https://doi.org/10.3168/jds.2006-513
Bradford M M. (1976). A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Analytical Biochemistry72(1–2): 248–254. https://doi.org/10.1016/0003-2697(76)90527-3
Brito, L F, Silva A E D F, Barbosa R T and Kastelic J P. (2004). Testicular thermoregulation in Bos indicus, crossbred and Bos Taurus bulls: Relationship with scrotal, testicular vascular cone and testicular morphology, and effects on semen quality and sperm production. Theriogenology 61(2–3): 511–528. https://doi.org/10.1016/S0093-691X(03)00231-0
Bustamante Filho I C. (2010). Clonagem, expressão e purificação de proteínas do plasma seminal bovino relacionadas à alta congelabilidade do sêmen [Cloning, expression and purification of the proteins from bovine seminal plasma osteopontin and acidic seminal fluid protein related with semen freezability]. [Doctoral diss., Universidade Federal do Rio Grande do Sul]. http://hdl.handle.net/10183/23020
Calvete J J, Mann K, Sanz L, Raida M and Töpfer-Petersen E. (1996). The primary structure of BSP-30K, a major lipid-, gelatin-, and heparin-binding glycoprotein of bovine seminal plasma. FEBS Letters 399(1–2): 147–152. https://doi.org/10.1016/S0014-5793(96)01310-5
CBRA. (2013). Manual para exame andrológico e avaliação de sêmen animal. 3rd ed. Belo Horizonte, Brazil: Colégio Brasileiro de Reprodução Animal (Brazilian College of Animal Reproduction).
Chenoweth, P J, Spitzer J C and Hopkins F M. (1992). A new bull breeding soundness evaluation form. In: Proceedings of the Annual Meeting of the Society for Theriogenology, San Antonio, 63–70.
Collodel G, Nerucci F, Signorini C, Iacoponi F and Moretti E. (2019). Associations between biochemical components of human semen with seminal conditions. Systems Biology in Reproductive Medicine 65(2): 155–163. https://doi.org/10.1080/19396368.2018.1548668
D’Amours O l, Frenette G, Fortier M, Leclerc P and Sullivan R. (2010). Proteomic comparison of detergent-extracted sperm proteins from bulls with different fertility indexes. Reproduction (Cambridge, England) 139(3): 545–556. https://doi.org/10.1530/REP-09-0375
Elzanaty S, Erenpreiss J and Becker C. (2007). Seminal plasma albumin: Origin and relation to the male reproductive parameters. Andrologia 39(2): 60–65. https://doi.org/10.1111/j.1439-0272.2007.00764.x
Façanha D A E, Ferreira J B, Morais Leite J H G, de Sousa J E R, Guilhermino M M, Costa W P, Bermejo Asensio L A, de Vasconcelos A M and Silveira R M F. (2019). The dynamic adaptation of Brazilian brahman bulls. Journal of Thermal Biology 81(April): 128–136. https://doi.org/10.1016/j.jtherbio.2019.02.016
Federación Colombiana de Ganaderos (FEDEGAN). (2021). Inventario Ganadero. https://www.fedegan.org.co/estadisticas/inventario-ganadero
Flowers W L. (2013). Triennial reproduction symposium: Sperm characteristics that limit success of fertilization. Journal of Animal Science 91(7): 3022–3029. https://doi.org/10.2527/jas.2012-5945
Fonseca V O, Franco C S and Bergmann J A G. (2000). Potencial reprodutivo e econômico de touros nelore acasalados coletivamente na proporção de um touro para 80 vacas. Arquivo Brasileiro de Medicina Veterinária e Zootecnia 52: 77–82. https:// doi.org/10.1590/S0102-09352000000100017
Franke D E. (1980). Breed and heterosis effects of American Zebu cattle. Journal of Animal Science 50(6): 1206–1214. https://doi.org/10.2527/jas1980.5061206x
Gerena R L, Irikura D, Urade Y, Eguchi N, Chapman D A and Killian G J. (1998). Identification of a fertility-associated protein in bull seminal plasma as lipocalin-type prostaglandin D synthase. Biology of Reproduction 58(3): 826–833. https://doi.org/10.1095/biolreprod58.3.826
Görg A and Weiss W. (2006). High-resolution two-dimensional electrophoresis with immobilized PH gradients for proteome analysis. In: Celis J E. (ed.), Cell biology: A laboratory handbook. Academic Press, 175–188. https://doi.org/10.1016/B978-012164730-8/50208-2
Hammond A C, Chase C C Jr, Bowers E J, Olson T A and Randel R D. (1998). Heat tolerance in Tuli-, Senepol-, and Brahman-sired F1 Angus heifers in Florida. Journal of Animal Science 76(6): 1568–1577. https://doi.org/10.2527/1998.7661568x
Killian G J, Chapman D A and Rogowski L A. (1993). Fertility-associated proteins in holstein bull seminal plasma. Biology of Reproduction 49(6): 1202–1207. https://doi.org/10.1095/biolreprod49.6.1202
Lees A M, Lees J C, Lisle A T, Sullivan M L and Gaughan J B. (2018). Effect of heat stress on rumen temperature of three breeds of cattle. International Journal of Biometeorology 62(2): 207–215. https://doi.org/10.1007/s00484-017-1442-x
Magalhães M J Jr, Martins L F, Senra R L, Santos T F, Okano D S, Pereira P R G, Faria- Campos A, Campos S V A, Guimarães J D and Baracat-Pereira M C. (2016). Differential abundances of four forms of binder of sperm 1 in the seminal plasma of Bos Taurus Indicus bulls with different patterns of semen freezability. Theriogenology 86(3): 766–777.e2. https://doi.org/10.1016/j.theriogenology.2016.02.030
Manjunath P and Therien I. (2002). Role of seminal plasma phospholipid-binding proteins in sperm membrane lipid modification that occurs during capacitation. Journal of Reproductive Immunology 53(1–2): 109–119. https://doi.org/10.1016/S0165-0378(01)00098-5
Menegassi S R O, Barcellos J O J, Lampert V D N, Borges J B S and Peripolli V. (2011). Bioeconomic impact of bull breeding soundness examination in cow-calf systems. Revista Brasileira de Zootecnia 40(2): 441–447. https://doi.org/10.1590/S1516-35982011000200028
Menezes E B, de Oliveira R V, van Tilburg M F, Barbosa E A, Nascimento N V, Velho A L M C S, Moreno F B, et al. (2017). Proteomic analysis of seminal plasma from locally-adapted ‘Curraleiro Pé-Duro bulls’ (Bos taurus): Identifying biomarkers involved in sperm physiology in endangered animals for conservation of biodiversity. Animal Reproduction Science 183(August): 86–101. https://doi.org/10.1016/j.anireprosci.2017.05.014
Merkies K, Chenier T, Plante C and Buhr M M. (2000). Assessment of stallion spermatozoa viability by flow cytometry and light microscope analysis. Theriogenology 54(8): 1215–1224. https://doi.org/10.1016/s0093-691x(00)00428-3
Meroni S B, Riera M F, Pellizzari E H and Cigorraga S B. (2002). Regulation of rat sertoli cell function by FSH: Possible role of phosphatidylinositol 3-kinase/protein kinase b pathway. The Journal of Endocrinology 174(2): 195–204. https://doi.org/10.1677/joe.0.1740195
Mortarino M, Tedeschi G, Negri A, Ceciliani F, Gottardi L, Maffeo G and Ronchi S. (1998). Two-dimensional polyacrylamide gel electrophoresis map of bull seminal plasma proteins. Electrophoresis 19(5): 797–801. https://doi.org/10.1002/elps.1150190532
Moura A A, Chapman D A, Koc H and Killian G J. (2006). Proteins of the cauda epididymal fluid associated with fertility of mature dairy bulls. Journal of Andrology 27(4): 534– 541. https://doi.org/10.2164/jandrol.05201
_________. (2007). A comprehensive proteomic analysis of the accessory sex gland fluid from mature holstein bulls. Animal Reproduction Science 98(3–4): 169–188. https://doi.org/10.1016/j.anireprosci.2006.03.012
Moura A A, Koc H, Chapman D A and Killian G J. (2006). Identification of proteins in the accessory sex gland fluid associated with fertility indexes of dairy bulls: A proteomic approach. Journal of Andrology 27(2): 201–211. https://doi.org/10.2164/jandrol.05089
O’Farrell P H. (1975). High resolution two-dimensional electrophoresis of proteins. The Journal of Biological Chemistry 250(10): 4007–4021. https://doi.org/10.1016/S0021-9258(19)41496-8
Rego J P, Crisp J M, Moura A A, Nouwens A S, Li Y, Venus B, Corbet N J, et al. (2014). Seminal plasma proteome of electroejaculated Bos indicus bulls. Animal Reproduction Science 148(1–2): 1–17. https://doi.org/10.1016/j.anireprosci.2014.04.016
Rocha D R, Martins J A M, van Tilburg M F, Oliveira R V, Moreno F B, Monteiro-Moreira A C O, Moreira R A, Araújo A A and Moura A A. (2015). Effect of increased testicular temperature on seminal plasma proteome of the ram. Theriogenology 84(8): 1291–1305. https://doi.org/10.1016/j.theriogenology.2015.07.008
Rodríguez-Martínez H. (2003). Laboratory semen assessment and prediction of fertility: Still Utopia? Reproduction in Domestic Animals 38(4): 312–318. https://doi.org/10.1046/j.1439-0531.2003.00436.x
Roncoletta M, Morani E S C, Esper C R, Barnabe V H and Franceschini P H. (2006). Fertility-associated proteins in nelore bull sperm membranes. Animal Reproduction Science 91(1–2): 77–87. https://doi.org/10.1016/j.anireprosci.2005.03.014
Sellem E, Broekhuijse M L W J, Chevrier L, Camugli S, Schmitt E, Schibler L and Koenen E P C. (2015). Use of combinations of in vitro quality assessments to predict fertility of bovine semen. Theriogenology 84(9): 1447–1454.e5. https://doi.org/10.1016/j.theriogenology.2015.07.035
Somashekar L, Selvaraju S, Parthipan S and Ravindra J P. (2015). Profiling of sperm proteins and association of sperm PDC-109 with bull fertility. Systems Biology in Reproductive Medicine 61(6): 376–387. https://doi.org/10.3109/19396368.2015.1094837
Valtorta S E, M R, Castro H C and Castelli M E. (1996). Artificial shade and supplementation effects on grazing dairy cows in Argentina. Transactions of the ASAE 39(1): 233– 236. https://doi.org/10.13031/2013.27503
van Tilburg M F, Salles M G, Silva M M, Moreira R A, Moreno F B, Monteiro-Moreira A C, Martins J A, Candido M J, Araujo A A and Moura A A. (2014). Semen variables and sperm membrane protein profile of Saanen bucks (Capra hircus) in dry and rainy seasons of the Northeastern Brazil (3°S). International Journal of Biometeorology 59: 561–573. https://doi.org/10.1007/s00484-014-0869-6
Yang Y, Yang S, Tang J, Ren G, Shen J, Huang B, Lei C, Chen H and Qu K. (2022). Comparisons of hematological and biochemical profiles in Brahman and Yunling cattle. Animals 12(14): 1813. https://doi.org/10.3390/ani12141813