Leaf Architecture and Genome Size Variation of Durio zibethinus L. from Jelebu, Negeri Sembilan, Malaysia

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Kamaruddin Shamin-Shazwan
Rozilawati Shahari
Che Nurul Aini Che Amri
Mohd Razik Midin


Durio zibethinus L. is known as the “king of fruit” in Malaysia. Meanwhile, Jelebu, Negeri Sembilan has always become the top choice district to visit for durian lover for its Durian Kampung Jelebu, which possessed good quality on par with top D. zibethinus clones such as D197 Musang King and D24. However, there is still lacking in taxonomic data of D. zibethinus especially from Jelebu. This study aimed to analyse the leaf architecture and genome size variations of selected D. zibethinus accessions from Jelebu. Five D. zibethinus accessions from Jelebu were examined. Thirty-seven parameters of gross leaf morphological characteristics and leaf venation pattern were observed and recorded for identification and classification of D. zibethinus accessions from Jelebu. Seven parameters have been recorded which are petiole length, petiole features, leaf size, leaf shape, leaf base shape, lower leaf surface colour and areolation could be used in differentiating between accessions. Results of this study showed the intraspecific variations existed among D. zibethinus accessions from Jelebu with a genome size varying between 1.7433 pg and 1.800 pg. In conclusion, data on leaf architecture and genome size variations from D. zibethinus accessions are beneficial for early plant identification and classification.

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Leaf Architecture and Genome Size Variation of Durio zibethinus L. from Jelebu, Negeri Sembilan, Malaysia. (2024). Tropical Life Sciences Research, 35(1), 177–191. https://doi.org/10.21315/tlsr2024.35.1.10
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Ackerfield J and Wen J A. (2002). Morphometric analysis of Hedera L. (the ivy genus, Araliaceae) and its taxonomic implications. Adansonia 24(2): 197–212.

Ardiyani M. (2015). A new species of Zingiber (Zingiberaceae) from Enggano Island, Indonesia. Reinwardtia 14(2): 307–310. https://doi.org/10.14203/reinwardtia.v14i2.1676

Bernama (2018). LPP identifies potential area for durian cultivation. https://www.malaymail.com/news/malaysia/2018/01/10/lpp-identifies-potential-area-for-durian-cultivation/1550837 (accessed on 8 June 2020).

Bulger N (2017). Botanical project: Leaf skeletons (non toxic). https://www.naomiloves.com/blog/2017/11/19/how-to-make-leaf-skeletons-non-toxic (accessed on 10 November 2020).

Cavallini A and Natali L. (1991). Intraspecific variation of nuclear DNA content in plant species. Caryologia 44(1): 93–107. https://doi.org/10.1080/00087114.1991.10797023

Department of Agriculture Malaysia (DOA). (2020). Garis panduan kebangsaan untuk menjalankan ujian kelainan, keseragaman dan kestabilan. http://pvpbkkt.doa.gov.my/Test%20Guidelines/Durian.pdf (accessed on 14 July 2020).

DOA. (2013). Durian. Pulau Pinang: Department of Agriculture. http://jpn.penang.gov.my/index.php/perkhidmatan/teknologi-tanaman/buah-buahan/64-durian-sp-26113 (accessed on 10 November 2020).

Effendi L N. (2013). Identifikasi morfologi durian Durio zibethinus Sunan dan Brongkol dalam penyusunan basis data keragaman. Masters diss., Universitas Sebelas Maret, Indonesia.

Ellis B, Daly D, Hickey L J, Johnson K, Mitchell J D, Wilf P and Wing S L. (2009). Manual of leaf architecture. Washington DC: Leaf Architecture Working Group. https://doi.org/10.1079/9781845935849.0000

Hahn P G and Maron J L. (2016). A framework for predicting intraspecific variation in plant defense. Trends in Ecology and Evolution 31(8): 646–656. https://doi.org/10.1016/j.tree.2016.05.007

Hernandez J O, Maldia L S J, Pulan D E, Buot I E and Park B B. (2020). Leaf architecture and petiole anatomy of Philippine Dipterocarpus species (Dipterocarpaceae). Bangladesh Journal of Plant Taxonomy 27(1): 1–14. https://doi.org/10.3329/bjpt.v27i1.47564

Hussain M, Azhar F M and Khan A A. (2008). Genetic basis of variation in leaf area, petiole length and seed cotton yield in some Cotton (Gossypium hirsutum) genotypes. International Journal of Agriculture and Biology 10: 705–708.

Ibrahim F S, Amon Z, Dasiman R and Anuar N A. (2022). Review on pharmacological properties of Christia vespertilionis. Malaysian Journal of Medicine and Health Sciences 18(5): 200–207. https://doi.org/10.47836/mjmhs.18.5.27

Idris S. (2011). Durio of Malaysia. Malaysia: Malaysian Agricultural Research and Development Institute.

Jarret R L, Ozias-Akins P, Phatak S, Nadimpalli R, Duncan R and Hiliard S. (1995). DNA contents in Paspalum spp. determined by flow cytometry. Genetic Resources and Crop Evolution 42: 237–242. https://doi.org/10.1007/BF02431258

Jennings K. (2019). Durian fruit: Smelly but incredibly nutritious. https://www.healthline.com/nutrition/durian-fruit (accessed on 15 November 2019).

Kandaiah V, Singaram N and Kandasamy K I. (2021). Optimized flow cytometric protocol and genome size estimation of Sabah snake grass (Clinacanthus nutans). Journal of Medicinal Plants Research 15(12): 531–539. https://doi.org/10.5897/JMPR2021.7162

Laraño A A P and Buot Jr I E. (2010). Leaf architecture of selected species of Malvaceae sensu APG and its taxonomic significance. Philippine Journal of Systematic Biology 4: 21–54. https://doi.org/10.3860/pjsb.v4i0.1563

Liu B B and Hong D Y A. (2016). Taxonomic revision of the Pourthiaea villosa complex (Rosaceae). Phytotaxa 244(3): 201–247. https://doi.org/10.11646/phytotaxa.244.3.1

Midin M R, Fikri M I and Zailani S S. (2020). Nuclear genome size determination of Christia vespertilionis via flow cytometry. Acta Chemica Malaysia 4(2): 72–75. https://doi.org/10.2478/acmy-2020-0012

Mishra M K, Padmajyothi D, Prakash N S, Ram A S, Srinivasan C S and Sreenivasan M S. (2010). Leaf architecture in Indian Coffee (Coffea arabica L.) cultivars and their adaptive significance. World Journal of Fungal and Plant Biology 1(2): 37–41.

Morton J F. (1987). Durian. In Fruits of warm climates. Miami: Purdue Edu, 287–291. O’Gara E, Guest D I and Hassan N M. (2004). Botany and production of durian (Durio zibethinus) in Southeast Asia. In A Drenth and D I Guest (eds.), Diversity and management of Phytophtora in Southeast Asia. Canberra, Australia: Australian Centre for International Agricultural Research, ACIAR Monograph, 180–186.

Oliveira E F, Bezerra D G, Santos M L, Rezende M H and Paula J A M. (2017). Leaf morphology and venation of Psidium species from the Brazilian Savanna. Revista Brasileira de Farmacognosia 27: 407–413. https://doi.org/10.1016/j.bjp.2017.03.005

Pélabon C, Hilde C H, Einum S and Gamelon M. (2020). On the use of the coefficient of variation to quantify and compare trait variation. Evolution Letters 4(3): 180–188. https://doi.org/10.1002/evl3.171

Pratiwi N, Hanafiah D S and Siregar L A M. (2018). Identifikasi karakter morfologis durian (Durio zibethinus Murr) di Kecamatan Tigalingga dan Pegagan Hilir Kabupaten Dairi Sumatera Utara. Jurnal Agroekoteknologi FP USU 6(2): 200–208.

Roches S D, Post D M, Turley N E, Balley J K, Hendry A P, Kinnison M T, Schweitzer J A and Palkovacs E P. (2018). The ecological importance of intraspecific variation. Nature Ecology and Evolution 2(1): 57–64. https://doi.org/10.1038/s41559-017-0402-5

Salma I. (1999). The taxonomic significance of trichome morphology in the genus Durio (Bombacaceae). The Garden’s Bulletin Singapore 51(4): 55–70.

Seshagirirao K, Harikrishnanaik L, Venumadhav K, Nanibabu B, Jamir K, Ratnamma B K, Jena R and Babarao D K (2016). Preparation of herbarium specimen for plant identification and voucher number. Roxburgia 6(1–4): 111–119.

Shan Y, Deng C, Hu W, Chen J, Chen X, Zheng S and Qin Q. (2019). First insight into diversity of leaf colour of loquat (Eribotrya) and its potential value on taxonomy. Genetic Resources and Crop Evolution 66: 143–163. https://doi.org/10.1007/s10722-018-0702-x

Siddiq M and Nasir M. (2012). Dragon fruit and durian. In M Siddiq (ed.). Tropical and subtropical fruits: Postharvest physiology, processing and packaging, New York: John Wiley & Sons Inc, 587–596. https://doi.org/10.1002/9781118324097.ch30

Siew G Y, Ng W L, Salleh M F, Tan S W, Ky H, Alitheen N B M, Tan S G and Yeap S K. (2018). Assessment of the genetic variation of Malaysian durian varieties using inter-simple sequence repeat markers and chloroplast DNA sequences. Tropical Agriculture Science 41(1): 321–332.

Smarda P and Bures P. (2006). Intraspecific DNA content variability in Festuca pallens on different geographical scales and ploidy levels. Annals of Botany 98: 665–678. https://doi.org/10.1093/aob/mcl150

Soegeng-Reksodihardjo W. (1962). The species of Durio with edible fruits. Economic Botany 16(4): 270–282. https://doi.org/10.1007/BF02860185

Spinner N B and Ferguson-Smith M A. (2019). Chapter 5: Cytogenetic analysis. In R E Pyeritz, B R Korf and W W Grody (eds.). Emery and Rimoin’s principles and practice of medical genetics and genomics, 7th ed. Cambridge: Academic Press, 145–164. https://doi.org/10.1016/B978-0-12-812536-6.00005-5

Sundari. (2015). Morphological variation of local durian (Durio zibethinus Murr.) on the Ternate Island. In K Muzakhar, Purwatiningsih, E T Utami, Siswoyo, F B Ulum, R Setiawan, S Ubaidillah, A Barokah, Z Khoiriyah and A Jannah. (eds.). Proceeding of the International Conference on Life Science and Biotechnology (ICOLIB): Exploration and Conservation of Biodiversity. Indonesia: ICOLIB, 143–148.

Talip N and Shamsuddin N S. (2019). Bombacaceae: Ciri anatomi dan mikromorfologi daun, 1st ed. Kuala Lumpur, Malaysia: Dewan Bahasa dan Pustaka.

Teh B T, Lim K, Yong C H, Ng C C Y, Rao S R, Rajasegaran V, Lim W K, Ong C K, Chan K, Cheng V K Y, Soh P S, Swarup S, Rozen S G, Nagarajan N and Tan P. (2017). The draft genome of tropical fruit durian (Durio zibethinus). Nature Genetics 49(11): 1633–1644. https://doi.org/10.1038/ng.3972

Temsch E M, Koutecky P, Urfus T, Smarda P and Dolezel J. (2021). Reference standards for flow cytometric estimation of absolute nuclear DNA content in plants. Cytometry 101: 710–724. https://doi.org/10.1002/cyto.a.24495