Moulting Performances Evaluation of Female Orange Mud Crab, Scylla olivacea (Herbst, 1796) In-Captivity: Effects of Water Salinity and Limb Autotomy
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Abstract
Female Scylla olivacea has become more popular in Malaysia as emerging species mainly for soft-shell crabs and crab fattening (to increase weight, size and ovary maturation so that they can be sold at a higher price). To harvest crabs in soft-shell conditions and fattening, both conditions depend mostly on moulting events. To accelerate the moulting process, the manipulation of water parameter (salinity) and autotomy of the limb is commonly used. In this study, the evaluation of the moulting performances of full limb autotomy (the removal of all the appendages except for the swimming legs) and non-ablated (control) using immature S. olivacea cultured in three different salinity treatments (10 ppt, 20 ppt and 30 ppt) were performed. Results indicate there were significant differences between mud crab’s culture duration, BW increments, growth performances and feeding efficiency with salinity. However, CW increments and survival indicate no significant effect with salinity. Meanwhile, limb autotomy proved to affect the culture duration, BW increments, survival and feeding efficiency of S. olivacea. The study concludes that both salinity and limb autotomy play significant roles in moulting performances of S. olivacea, with 20 ppt being the best salinity to stimulate S. olivacea moulting and development compared with the other two treatments (10 ppt and 30 ppt). Limb autotomy also indicates promising results as this technique proved to accelerate the moulting duration of S. olivacea with a 100% moulting percentage within 30 days. Therefore, the outcome would certainly benefit in the aquaculture production of this species of commercial importance mainly on soft-shell crabs production and also emerge as crabs fattening technique.
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References
Alberts-Hubatsch H, Lee S Y, Jan-Olaf M, Diele K, Nordhaus I and Wolff M. (2016). Life history, movement, and habitat use of Scylla serrata (Decapoda: Portunidae) current knowledge and future challenges. Hydrobiologia 763: 5–21. https://doi.org/10.1007/s10750-015-2393-z
Amin-Safwan A, Muhd-Farouk H, Hasyima-Ismail N, Mahsol H H and Ikhwanuddin M. (2019a). Relationship between the carapace width and body weight increments and the confirmation of Stage 1 ovary after the molting of immature orange mud crab, Scylla olivacea, (Herbst, 1796) in captivity. Songklanakarin Journal of Science and Technology 41(6): 1226–1232.
Amin-Safwan A, Muhd-Farouk H, Mardhiyyah M P, Nadirah M and Ikhwanuddin M. (2019b). Does water salinity affect the level of 17β-estradiol and ovarian physiology of orange mud crab, Scylla olivacea (Herbst, 1796) in captivity? Journal of King Saud University-Science 31: 827–835. https://doi.org/10.1016/j.jksus.2018.08.006
Amin-Safwan A, Muhd-Farouk H, Nadirah M and Ikhwanuddin M. (2016). Effect of water salinity on the external morphology of ovarian maturation stages of orange mud crab, Scylla olivacea (Herbst, 1796) in captivity. Pakistan Journal of Biological Sciences 19: 219–226. https://doi.org/10.3923/pjbs.2016.219.226
Aslamyah S and Fujaya Y. (2010). Stimulasi molting dan pertumbuhan kepiting bakau (Scylla sp.) melalui aplikasi pakan buatan berbahan dasar limbah pangan yang diperkaya dengan ekstrak bayam. Ilmu Kelautan 15(3): 170–178.
Association for the Study of Animal Behaviour (ASAB) (2012). Guidelines for the treatment of animals in behavioural research and teaching. Animal Behaviour 83: 301–309.
Baylon J C. (2013). The combined effects of salinity and temperature on the survival and development of zoea, megalopa and crab instar larvae of mud crab, Scylla tranquebarica (Fabricius 1798). Asian Fisheries Science 26: 14–25. https://doi.org/10.33997/j.afs.2013.26.1.002
Bennett D B. (1973). The effect of limb loss and regeneration on growth of the edible crab, Cancer pagurus Linnaeus, 1758. Journal of Experimental Marine Biology and Ecology 13: 45–53. https://doi.org/10.1016/0022-0981(73)90045-2
Browser P R and Rosemark R. (1981). Mortalities of cultured lobster, Homarus, associated with a molt death syndrome. Aquaculture 23(1–4): 11–18. https://doi.org/10.1016/0044-8486(81)90003-X
Chand B K, Trivedi R K, Dubey S K, Rout S K, Beg M M and Das U K. (2015). Effect of salinity on survival and growth of giant freshwater prawn Macrobrachium rosenbergii (de Man). Aquaculture Reports 2: 26–33. https://doi.org/10.1016/j.aqrep.2015.05.002
Chang E S and Mykles D L. (2011). Regulation of crustacean molting: A review and our perspectives. General and Comparative Endocrinology 172(3): 323–330. https://doi.org/10.1016/j.ygcen.2011.04.003
Chazaro-Olvera S and Peterson M S. (2004). Effect of salinity on growth and molting of sympatric Callinectes spp. from Camaronera Lagoon, Veracruz, Mexico. Bulletin of Marine Science 74(1): 115–127.
Darnell M Z, Rittschof C C, Rittschof J, Beach C and Rittschof D. (2018). Autotomy of the major claw stimulates molting and suppresses feeding in fiddler crabs. Journal of Experimental Marine Biology and Ecology 509: 66–70.
https://doi.org/10.1016/j.jembe.2018.09.001
Fazhan H, Waiho K, Darin Azri M F, Al-Hafiz I, Wan Norfaizza W I, Megat F H, Jasmani S, Ma H and Ikhwanuddin M. (2017). Sympatric occurrence and population dynamics of Scylla spp. in equatorial climate: Effects of rainfall, temperature and lunar phase. Estuarine, Coastal and Shelf Science 198: 299–310. https://doi.org/10.1016/j. ecss.2017.09.022
Fingerman M and Fingerman S W. (1974). The effect of limb removal on the rates of ecdysis of eyed and eye stalkless crabs, Uca pugilator. Zoologische Jahrbucher Physiology 78: 301–309.
Fishery Bureau of Ministry of Agriculture of China. (2012). China fisheries yearbook. Chinese Agricultural Press, China, Beijing.
Food and Agriculture Organization of the United Nations (FAO) (2018). Species fact sheets, Scylla serrata. http://www.fao.org/fishery/species/2637/en (accessed on 5 January 2022).
Fujaya Y. (2011). Growth and molting of mud crab administered by different doses of vitomolt. Jurnal Akuakultur Indonesia 10(1): 24–28. https://doi.org/10.19027/jai.10.24-28
Fujaya Y, Rukminasari N, Alam N, Rusdi M, Fazhan H and Waiho K. (2020). Is limb autotomy really efficient compared to traditional rearing in soft-shell crab (Scylla olivacea) production? Aquaculture Reports 18: 100432. https://doi.org/10.1016/j.aqrep.2020.100432
Gaude A R and Anderson J A. (2011). Soft shell crab shedding systems. Southern Regional Aquaculture Center 4306: 1–6.
Gong H, Jiang D H, Lightner D V, Collins C and Brock D. (2008). A dietary modification approach to improve the osmoregulatory capacity of Litopenaeus vannamei cultured in the Arizona desert. Aquaculture Nutrition 10: 227–236. https://doi.org/10.1111/j.1365-2095.2004.00294.x
Gong J, Yu K, Shu L, Ye H, Li S and Zeng C. (2015). Evaluating the effects of temperature, salinity, starvation and autotomy on molting interval and expression of ecdysone receptor in early juvenile mud crabs, Scylla paramamosain. Journal of Experimental Marine Biology and Ecology 464: 11–17. https://doi.org/10.1016/j.jembe.2014.12.008
Hartnoll R G. (1982). Growth. In L G Abele (ed.). The biology of crustacea, embryology, morphology and genetics. New York: Academic Press, 111–185.
He J, Shao L C, Hong Y H, Lu Y, Yang X Z and Cheng Y X. (2013). Impacts of starvation on the juveniles of Chinese mitten crab Eriocheir sinensis. Chinese Journal of Ecology 32: 2077–2084.
He J, Wu X and Cheng Y. (2016). Effects of limb autotomy on growth, feeding and regeneration in the juvenile Eriocheir sinensis. Aquaculture 457: 79–84. https://doi.org/10.1016/j.aquaculture.2016.02.004
Hopkins P M. (1982). Growth and regeneration patterns in the fiddler crab, Uca pugilator. The Biological Bulletin 163(2): 301–319. https://doi.org/10.2307/1541268
Hosamani N, Pamuru R R and Pamanji S R. (2016). Natural and induced (eyestalk). Journal of Aquaculture Research and Development 7: 424.
Ikhwanuddin M, Azmie G, Juariah H M, Zakaria M Z and Ambak M A. (2011). Biological information and population features of mud crab, genus Scylla from mangrove areas of Sarawak, Malaysia. Fisheries Research 108: 299–306. https://doi.org/10.1016/j.fishres.2011.01.001
Ikhwanuddin M, Bachok Z, Mohd-Faizal W W Y, Azmie G and Abol-Munafi A B. (2010). Size of maturity of mud crab Scylla olivacea (Herbst, 1796) from mangrove areas of Terengganu coastal waters. Journal of Sustainability Science and Management 5: 134–147.
Ikhwanuddin M, Noor-Baiduri S, Wan-Norfaizza W I and Abol-Munafi A B. (2014). Effect of water salinity on mating success of orange mud crab, Scylla olivacea (Herbst, 1796) in captivity. Journal of Fisheries and Aquatic Science 9(3): 134–140. https://doi.org/10.3923/jfas.2014.134.140
Jantrarotai P, Taweechuer K and Pripanapong S. (2002). Salinity levels on survival rate and development of mud crab (Scylla olivacea) from zoea to megalopa and from megalopa to crab stage. Kasetsart Journal – Natural Science 36: 278–284.
Juanes F and Smith L D. (1995). The ecological consequences of limb damage and loss in decapod crustaceans: a review and prospectus. Journal of Experimental Marine Biology and Ecology 193: 197–223.
https://doi.org/10.1016/0022-0981(95)00118-2
Keenan C P. (1999). Culture of the mud crab, Genus Scylla: Past, present and future. In C P Keenan and A A Blackshaw (eds.). Mud crab aquaculture and biology. Proceedings of an international scientific forum held in Darwin, Australia, 21–24 April 1997. ACIAR Proceedings 78, 216.
Keenan C P, Davie P J F and Mann D L. (1998). A revision of the genus Scylla De Haan, 1833 (Crustacea: Decapoda: Brachyura: Portunidae). Raffles Bulletin of Zoology 46: 217–245.
La Sara. (2001). Habitat and some biological parameters of two species of mud crab Scylla in Southeast Sulawesi, Indonesia. In O Carman, A Sulistiono, T Purbayanto, S S Watanabe and T Arimoto (eds.). TUF International JSPS Project Vol. 10. Proceedings JSPS DGHE International Symposium of Fisheries Science in Tropical Area. Konan Minatoku, Tokyo, Japan, 341–346.
Lemos D, Phan V N and Alvarez G. (2001). Growth, oxygen consumption, ammonia-N excretion, biochemical composition and energy content of Farfantepenaeus paulensis Perez-Farfante (Crustacea, Decapoda, Penaeidae) early postlarvae in different salinities. Journal of Experimental Marine Biology and Ecology 261(1): 55–74. https://doi.org/10.1016/S0022-0981(01)00260-X
Ma Y C, Yang Y J and Wang G L. (2013). Effects of salinity challenge on the immune factors of Scylla serrata. Acta Agricultural Zhejiangensis 22: 479–484.
Madsen T and Shine R. (1992). Determinants of reproductive success in female adders, Vipera berus. Oecologia 92(1): 40–47. https://doi.org/10.1007/BF00317260
Mariappan P, Balasundaram C and Schmitz B. (2000). Decapod crustacean chelipeds: An overview. Journal of Biosciences 25: 301–313. https://doi.org/10.1007/BF02703939
McCarthy J F and Skinner D M. (1977). Interruption of proecdysis by autotomy of partially regenerated limbs in the land crab, Gecarcinus lateralis. Developmental Biology 61: 299–310. https://doi.org/10.1016/0012-1606(77)90300-1
McGee E A, Hsu S Y, Kaipia A and Hsueh A J. (1998). Cell death and survival during ovarian follicle development. Molecular and Cellular Endocrinology 140(1–2): 15– 18. https://doi.org/10.1016/S0303-7207(98)00023-9
Mia M Y and Shah M M R. (2010). Effect of salinity on the survival and growth of mud crabling, Scylla serrata. University Journal of Zoology Rajshahi 29(1): 29–32. https://doi.org/10.3329/ujzru.v29i1.9462
Muhd-Farouk H, Nurul H A, Abol-Munafi A B, Mardhiyyah M P, Hasyima-Ismail N, Manan H, Fatihah S N, Amin-Safwan A and Ikhwanuddin M. (2019). Development of ovarian maturations in orange mud crab, Scylla olivacea (Herbst, 1796) through induction of eyestalk ablation and methyl farnesoate. Arab Journal of Basic and Applied Science 26(1): 171–181. https://doi.org/10.1080/25765299.2019.1588197
Mykles D L. (2001). Interaction between limb regeneration and molting in decapods crustacean. Integrative and Comparative Biology 41(3): 399–406. https://doi.org/10.1093/icb/41.3.399
Nadiah W N, Ikhwanuddin M and Abol-Munafi A B. (2012). Remarks on the mating behaviours and success of blue swimming crab, Portunus pelagicus (Linnaeus, 1766) through the induction of limb autotomy technique. Journal of Animal and Veterinary Advances 11(8): 1149–1157. https://doi.org/10.3923/javaa.2012.1149.1157
Parado-Estepa F D and Quinitio E T. (2011). Influence of salinity on survival and molting in early stages of three species of Scylla crabs. Israeli Journal of Aquaculture– Bamidgeh 63(IIC: 63.2011.631): 6.
Quinitio E T and Estepa F D P. (2011). Survival and growth of mud crab, Scylla serrata, juveniles subjected to removal or trimming of chelipeds. Aquaculture 318(1–2): 229–234. https://doi.org/10.1016/j.aquaculture.2011.05.034
Rahman M R, Asaduzzaman M, Zahangir M M, Islam S R, Nahid S A-A, Jahan D A, Mahmud Y and Khan M N A. (2020). Evaluation of limb autotomy as a promising strategy to improve production performances of mud crab (Scylla olivacea) in the soft-shell farming system. Aquaculture Research 51(6): 1–18. https://doi.org/10.1111/are.14598
Romano N and Zeng C. (2006). The effects of salinity on the survival, growth and haemolymph osmolality of early juvenile blue swimmer crabs, Portunus pelagicus. Aquaculture 260: 51–162. https://doi.org/10.1016/j.aquaculture.2006.06.019
Rouse D B and Kartamulia I. (1992). Influence of salinity and temperature on molting and survival of the Australia freshwater crayfish (Cherax tenuimanus). Aquaculture 105: 47–52. https://doi.org/10.1016/0044-8486(92)90160-M
Sang H M and Fotedar R. (2004). Growth, survival, haemolymph osmolality and organosomatic indices of the western king prawn (Penaeus latisulcatus Kishinouye, 1896) reared at different salinities. Aquaculture 234: 601–614. https://doi.org/10.1016/j.aquaculture.2004.01.008
Siti S. (2012). Effect of limb autotomy on moulting and growth of blue swimming crab, Portunus pelagicus for the production of soft shell crab. BSc diss., Faculty of Fisheries and Aqua-Industries, Universiti Malaysia Terengganu.
Skinner D M. (1985). Molting and regeneration. In D E Bliss and L H Mantel (eds.). The biology of crustacea. New York: Academic Press, 43–146. https://doi.org/10.1016/B978-0-12-106409-9.50013-0
Slos S, De Block M and Stoks R. (2008). Autotomy reduces immune function and antioxidant defence. Biology Letters 5: 90–92. https://doi.org/10.1098/rsbl.2008.0501
Talpur A D and Ikhwanuddin M. (2012). Effects of stress tests on larvae of blue swimming crab, Portunus pelagicus (Linnaeus, 1758). Advances in Environmental Biology 6: 1909–1915.
Vernberg W B, DeCoursey P J and Padgett W J. (1973). Synergistic effects of environmental variables on larvae of Uca pugilator. Marine Biology 22(4): 307–312. https://doi.org/10.1007/BF00391386
Waiho K, Fazhan H and Ikhwanuddin M. (2016). Size distribution, length-weight relationship and size at the onset of sexual of the orange mud crab, Scylla olivacea, in Malaysian waters. Marine Biology Research 12(7): 726–738. https://doi.org/10.1080/17451000.2016.1200726
Waiho K, Fazhan H, Baylon J C, Madihah H, Noorbairduri S, Ma H and Ikhwanuddin M. (2017). On types of sexual maturity in brachyurans, with special reference to size at the onset of sexual maturity. Journal of Shellfish Research 36(3): 807–839. https://doi.org/10.2983/035.036.0330
Waiho K, Fazhan H, Quinitio E T, Baylon J C, Fujaya Y, Azmie G, Wu Q, Shi X, Ikhwanuddin M and Ma H. (2018). Larval rearing of mud crab (Scylla): What lies ahead. Aquaculture 493: 37–50. https://doi.org/10.1016/j.aquaculture.2018.04.047
Wu Q, Shi X, Fang S, Xie Z, Guan M, Li S, Zheng H, Zhang Y, Ikhwanuddin M and Ma H. (2019). Different biochemical composition and nutritional value attribute to salinity and rearing period in male and female mud crab Scylla paramamosain. Aquaculture 513(15): 734417. https://doi.org/10.1016/j.aquaculture.2019.734417