Evaluating the Impact of Spinach Supplementation in Enhancing the Growth of Blue Swimming Crab Larvae, Portunus pelagicus
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Abstract
Blue swimming crabs (Portunus pelagicus) consistently command higher prices in domestic and global markets due to elevated demand. Due to the rising issue of low crab larvae survival, farmers have begun using artificial hormones to increase survival rates and ensure hatchery production aligns with market demands. However, the outcomes of using artificial hormones are concerning, as these compounds pose serious risks to aquatic organisms, humans, and the environment. To address the harmful effects of artificial hormones, efforts are now focused on identifying affordable and eco-friendly plant-based alternatives, such as spinach (Spinacia oleracea), which contains bioactive compounds that could promote crab larval growth and survival. This study aimed to investigate the efficacy of spinach in improving the growth performance of P. pelagicus larvae. This was accomplished by enriching Artemia with spinach, allowing it to serve as a nutrient-enhanced live feed. Compounds in the spinach leaf were extracted with methanol and analysed through GC-MS to identify potential growth-related compounds. LC50 analysis (1,000 ppm–2,000 ppm) was conducted on Artemia to determine the optimal enrichment concentration (1,600 ppm) before use in larval feeding. Results indicated spinach contains compounds such as steroids (5.9%), palmitic acid (5.2%), oleic acid (2.5%) and flavonoids (3.6%) that can potentially improve larval growth. Toxicity analysis showed that the 1,600 ppm treatment was the LC50, meaning it is the optimal concentration for Artemia enrichment, delivering the highest amount of bioactive compounds without affecting Artemia survival. Larvae were fed spinach-enriched Artemia (1,600 ppm) and compared with unenriched Artemia (control). The 1,600 ppm treatment resulted in significantly higher survival at various larval stages (Z1–Z2: 82.6%, Z2–Z3: 91.9%, M–C1: 38.1%), shorter intermoult periods for Z2–Z3 (2.9 days), Z3–Z4 (3.1 days), and M–C1 (3.8 days) and higher SGR (M: 22.5% day⁻¹, C1: 24.5% day⁻¹). Larval steroid analysis supported these findings, with treatment 1,600 ppm yielding higher steroid levels across all stages (511.63–3953 pg/mL), suggesting that spinach-derived steroids may stimulate moulting and enhance larval growth and survival.
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References
Alam N, Fujaya Y, Haryati H, Sari D K, Ahmad M, Rusdi M and Farizah N. (2019). The effect of Melastoma malabathricum leaf extract on growth and spawning of blue swimming crab (Portunus pelagicus). IOP Conference Series: Earth and Environmental Science 370(1): 012029–012029. https://doi.org/10.1088/1755-1315/370/1/012029
Ali M, Saade E and Tandipayuk H. (2023). Utilization of soursop leaf, Annona muricata, extract for improving growth performance and reduce cholesterol levels in fattening mangrove crab, Scylla olivacea. Journal of Pharmaceutical Negative Results 14(2): 804–814. https://doi.org/10.47750/pnr.2023.14.02.101
Ayotunde E O, Offem B O, Okey B I, Ikpi G U, Ochang S N, Agbam N E and Omini D O. (2010). Toxicity of pawpaw (Carica papaya) seed powder to sharptooth catfish Clarias gariepinus fingerlings and effects on haematological parameters. International Journal of Fisheries and Aquaculture 2(3): 71–78. https://doi.org/10.5897/ijfa.9000038
Baniesmaeili Y, Akbarzadeh A, Abdollahi F and Niroomand M. (2023). Effectiveness of dietary Moringa oleifera leaf powder and extract in the Pacific white shrimp (Litopenaeus vannamei). Iranian Journal of Fisheries Sciences 22(3): 547–565. https://doi.org/10.22092/ijfs.2023.129278
Duan B, Mu S, Guan Y, Li S, Yu Y, Liu W, Li Z, Ji X and Kang X. (2022). Genetic diversity and population structure of the swimming crab (Portunus trituberculatus) in China seas determined by genotyping-by-sequencing (GBS). Aquaculture 555: 738233. https://doi.org/10.1016/j.aquaculture.2022.738233
El-Refiae N M, Ayyat M S, Mahmoud H K and Naiel M A E. (2024). The effects of Paulownia leaf extract dietary administration on growth, redox status, immune responses, and modulate intestinal microbial content in Nile tilapia. Aquaculture International 32: 1857–1877. https://doi.org/10.1007/s10499-023-01247-9
Food and Agriculture Organization of the United Nations (FAO). (2022). The state of world fisheries and aquaculture 2022. Towards blue transformation. Rome: FAO. https://doi.org/10.4060/cc0461en
Fujaya Y, Trijuno D D, Haryati H, Hasnidar H, Rusdi M and Usman Z. (2019). Effectivity of mulberry leaf extract on stimulating ekdisteroid hemolymph content and molting of mud crab (Scylla olivacea). Torani 2(1): 32–43. https://doi.org/10.35911/torani.v2i1.5857
Hajar-Azira Z, Aaqillah-Amr M A, Rasdi N W, Ma H and Ikhwanuddin M. (2022). Preliminary investigation on the effect of fiddlehead fern, Diplazium esculentum, extract on the growth performance of giant freshwater prawn, Macrobrachium rosenbergii, postlarvae. Aquaculture International 31(1): 81–101. https://doi.org/10.1007/s10499-022-00965-w
Hidir A, Aaqillah-Amr M A, Mohd-Sabri M, Mohd-Zaidi I, Shahreza M S, Abualreesh M H, Peng T H, Ma H, Waiho K, Fazhan H, Mukti A T and Ikhwanuddin M. (2021). Thermal tolerance of purple mud crab, Scylla tranquebarica (Fabricius, 1798), during egg incubation, larval rearing and juveniles’ production. Aquaculture Research. https://doi.org/10.1111/are.15682
Ikhwanuddin M, Talpur A D, Azra M N, Mohd-Azlie B, Hii Y S and Abol-Munafi A B. (2012). Effects of stocking density on the survival, growth and development rate of early stage blue swimming crab, Portunus pelagicus (Linnaeus,
larvae. World Applied Science Journal 18(3): 379–384. https://doi.org/10.5829/idosi.wasj.2012.18.03.62156
Iromo H, Farizah N and Puryono. (2021). The application of thyroxine hormone and Melastoma malabathricum leaf extract as stimulators in gonadal maturation of Scylla serrata in traditional ponds. AACL Bioflux 14(3): 1769–1777.
Liao M, Wang F, Huang L, Liu C, Dong W, Zhuang X, Yin X, Liu Y and Wang W. (2023). Effects of dietary Ginkgo biloba leaf extract on growth performance, immunity and environmental stress tolerance of Penaeus vannamei. Fish Shellfish Immunology 132: 108500–108500. https://doi.org/10.1016/j.fsi.2022.108500
Lu Y P, Zheng P, Xu J R, Cao Y L, Li J, Hao C G, Zhang Z L, Xian J A, Zhang X and Wang A. (2023). Effects of dietary Eucommia ulmoides leaf extract on growth, muscle composition, hepatopancreas histology, immune responses
and microcystin-LR resistance of juvenile red claw crayfish (Cherax quadricarinatus). Fishes 8(1): 20. https://doi.org/10.3390/fishes8010020
Lutz C G. (2024). Crabs. In M Dikeman (ed.), Encyclopedia of meat sciences, 3rd ed., Academic Press, 40–49. https://doi.org/10.1016/b978-0-323-85125-1.00124-1
Naviah S, Huda S and Arini D. (2022). Measured catch of blue swimming crab (Portunus pelagicus) based on the Walter-Hilbron non-equilibrium model in Bangkalan Madura waters. Indonesian Journal of Tropical Aquatic 5(2): 124–141. https://doi.org/10.22219/ijota.v5i2.24292
Natnan M E, Low C F, Chong C M, Daud N I N A A, Om A D and Baharum S N. (2022). Comparison of different dietary fatty acids supplement on the immune response of hybrid grouper (Epinephelus fuscoguttatus × Epinephelus
lanceolatus) challenged with Vibrio vulnificus. Biology 11(9): 1288. https://doi.org/10.3390/biology11091288
Huda-Faujan N, Zubairi S I and Baker A N. (2023). Nutritional and bioactive constituents of antioxidant and antimicrobial properties in Spinacia oleracea: A review. Sains Malaysiana 52(9): 2571–2585. https://doi.org/10.17576/jsm-2023-5209-08
Saptiani G, Prayitno S B and Anggarawati S. (2021). Effect of mangrove leaf extract (Acanthus ilicifolius) on non-specific immune status and vibriosis resistance of black tiger shrimps (Penaeus monodon) challenged with Vibrio harveyi. Veterinary World 14(8): 2282–2289. https://doi.org/10.14202/vetworld.2021.2282-2289
Sorach K, Pratoomchat B, Hanna P J and Suksamrarn A. (2013). Effect of phytoecdysone on the molting period and survival rate of the blue swimming crab, Portunus pelagicus. Journal of Science Technology 11(2): 87–94.
Sugumar V, Vijayalakshmi G and Saranya K. (2013). Molt cycle related changes and effect of short-term starvation on the biochemical constituents of the blue swimmer crab Portunus pelagicus. Saudi Journal Biological Science 20(1): 93–103. https://doi.org/10.1016/j.sjbs.2012.10.003
Yu Y, Li C, Liu J, Zhu F, Wei S, Huang Y, Huang X and Qin Q. (2020). Palmitic acid promotes virus replication in fish cell by modulating autophagy flux and TBK1-IRF3/7 pathway. Frontier Immunology 11: 1764. https://doi.org/10.3389/fimmu.2020.01764
Yue Y, Ma H L, Cheng C H, Liu G X, Fan S G, Jiang J J and Guo Z X. (2023). Effects of dietary guava leaf aqueous extract supplementation on growth, antioxidant capacity, and non-specific immunity in mud crab Scylla paramamosain. Israeli Journal of Aquaculture - Bamidgeh 75. https://doi.org/10.46989/001c.77654