Anti-diabetic and Antioxidant Activities of a Bornean Ethnomedicinal Plant: In vitro and In silico Investigations of Fibraurea tinctoria Root Fractions

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Published: 15-10-2026
DOI: https://doi.org/10.21315/tlsr2026.37.3.10
Keywords:
3-Isoxazolecarboperoxoic Acid, Antioxidant, Anti-diabetic, Ethnomedicine, Borneo, Empirical Investigations

Main Article Content

Yudi Purnomo
Department of Pharmacy, Faculty of Medicine, University of Islam Malang, Malang, Indonesia
Apon Zaenal Mustopa
Research Center for Genetic Engineering, Research Organization for Life Science and Environment, National Research and Innovation Agency (BRIN), KST Soekarno, Cibinong, Indonesia
Jendri Mamangkey
Department of Biology, Faculty of Mathematics and Natural Sciences, Universitas Negeri Makasar, Makasar, Indonesia
Herman Irawan
Research Center for Genetic Engineering, Research Organization for Life Science and Environment, National Research and Innovation Agency (BRIN), KST Soekarno, Cibinong, Indonesia

Abstract

Fibraurea tinctoria, a native ethnomedicinal plant from Borneo, has been traditionally used to treat diabetes, with preclinical studies suggesting anti-hyperglycemic activity. However, the mechanisms underlying its hypoglycemic effects and active compounds remain unclear. This study aims to evaluate the anti-diabetic potential of F. tinctoria root extract through in vitro α-glucosidase inhibition and antioxidant assays, supported by in silico analysis. The roots were extracted using methanol, followed by fractionation with n-hexane (Fraction C6), ethyl acetate (Fraction EtOAc), n-butanol (Fraction BuOH) and aqueous solvent (Fraction H2O). The α-glucosidase inhibition assay was conducted using p-nitrophenyl-α-D-glucopyranoside (p-NPG), and antioxidant activity was measured using the DPPH method. Data were presented as mean ± SD, with IC50 values calculated by linear regression. Fraction EtOAc exhibited the strongest inhibitory activity against both α-glucosidase (IC50 = 147 ± 3.18 μg/mL) and free radicals (IC50 = 155 ± 2.30 μg/mL) compared with other fractions, though lower than reference standard acarbose and ascorbic acid. Phytochemical analysis using GC-MS identified bioactive compounds, further confirmed by molecular docking. 3-Isoxazolecarboperoxoic acid demonstrated optimal interactions with α-glucosidase (3TOP) and the aldose reductase (1IEI). These findings suggest that F. tinctoria could be a promising source for novel anti-diabetic and antioxidant agents.

Article Details

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Vol. 37 No. 3 (2026)

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Original Article
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This work is licensed under a Creative Commons Attribution 4.0 International License.

How to Cite

Yudi Purnomo, Apon Zaenal Mustopa, Jendri Mamangkey, & Herman Irawan. (2026). Anti-diabetic and Antioxidant Activities of a Bornean Ethnomedicinal Plant: In vitro and In silico Investigations of Fibraurea tinctoria Root Fractions. Tropical Life Sciences Research, 37(3), 209-232. https://doi.org/10.21315/tlsr2026.37.3.10

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