Determination of propranolol concentration in human plasma by high-performance liquid chromatography

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Published: Jan 31, 1999
DOI: https://doi.org/10.21315/

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Zain-Hamid R
Department of Pharmacology, Department of Medicine, The School of Medical Sciences, Universiti Sains Malaysia, 16150 Kubang Kerian, Kelantan
Ismail Z
Department of Pharmacology, Department of Medicine, The School of Medical Sciences, Universiti Sains Malaysia, 16150 Kubang Kerian, Kelantan
Mahendra Raj S
Department of Pharmacology, Department of Medicine, The School of Medical Sciences, Universiti Sains Malaysia, 16150 Kubang Kerian, Kelantan
Mohsin S.S. J.
Department of Pharmacology, Department of Medicine, The School of Medical Sciences, Universiti Sains Malaysia, 16150 Kubang Kerian, Kelantan

Abstract

The concentration of propranolol in plasma was determined by HPLC using Lichrosorb reverse phase column C18 (Phenomenex) with UV detection at 295 nm and oxprenolol as an internal standard. Run time for each analysis was 10 minutes with recovery of 92.3% to 103.6%. Intra-day and inter-day precision expressed as a mean percent of coefficient of variation (CV) were 6.41% and 3.03% respectively. The accuracy of intra-day and inter day assays were 2.97% and 2.87% respectively. Propranolol in plasma kept frozen at -200C was stable within 6 weeks. The analysis of propranolol concentration was later used to study the pharmacokinetics of propranolol in cirrhotic Malay patients.

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1.
Zain-Hamid R, Ismail Z, Mahendra Raj S, Mohsin S.S. J. Determination of propranolol concentration in human plasma by high-performance liquid chromatography. Malays J Med Sci [Internet]. 1999 Jan. 31 [cited 2026 Oct. 18];6(1):61-6. Available from: https://ejournal.usm.my/mjms/article/view/mjms_vol6-no-1-1999_10
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Vol. 6 No. 1 (1999): Abstracts
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Abstract
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