ENHANCEMENT OF LIGNIN PEROXIDASE AND MANGANESE PEROXIDASE PRODUCTION BY HUMICOLA GRISEA IN A TUBULAR AIR-LIFT FERMENTER

Abstract

The production of lignin peroxidase (LiP) and manganese peroxidase (MnP) by Humicola grisea in a tubular air-lift fermenter were studied. The optimized production parameters used in the fermenter were aeration rate of 1.0 vvm, 1.0% (w/v) glucose, 10.5 mM (NH₄)₂HPO₄ and inoculum concentration of 1.0% (v/v; 6 × 10⁶ spores/ml). The increment of about 52% of LiP and 49% of MnP productions were obtained after optimization of the parameters.

Penghasilan enzim lignin peroksidase (LiP) dan mangan peroksidase (MnP) oleh Humicola grisea di dalam fermenter angkut udara diselidiki. Penghasilan optimum parameter yang digunakan pada fermenter ini ialah kadar pengudaraan 1.0 vvm, 1.0% (b/i) glukosa, 10.5 mM (NH₄)₂HPO₄ dan kepekatan inokulum 1.0% (i/i; 6 × 10⁶ spora/ml). Peningkatan sebanyak 52% dalam penghasilan LiP dan 49% dalam MnP diperoleh selepas semua parameter dioptimumkan.